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MYO9B Polyclonal Antibody

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Antibody Testing Data (6)

MYO9B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

MYO9B Antibody (12432-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 12432-1-AP (MYO9B antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

MYO9B Antibody in Immunoprecipitation (IP)

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Product Details

12432-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MYO9B Fusion Protein Ag3108 (1660-1983 aa encoded by BC018108)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DKALLCSVC KMTCHKKCVH KIQSHCSYTY GRKGEPGVEP GHFGVCVDSL TSDKASVPIV LEKLLEHVEM HGLYTEGLYR KSGAANRTRE LRQALQTDPA AVKLENFPIH AITGVLKQWL RELPEPLMTF AQYGDFLRAV ELPEKQEQLA AIYAVLEHLP EANHNSLERL IFHLVKVALL EDVNRMSPGA LAIIFAPCLL RCPDNSDPLT SMKDVLKITT CVEMLIKEQM RKYKVKMEEI SQLEAAESIA FRRLSLLRQN APWPLKLGFS SPYEGVLNKS PKTRDIQEEE LEVLLEEEAA GGDEDREKEI LIERIQSIKE EKEDI (1660-1983 aa encoded by BC018108 )

Target Information

This gene encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. The protein has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. The protein complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. The protein also has rho-GTPase activity. Polymorphisms in this gene are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mutations in MYO9B are associated with Charcot-Marie-Tooth disease type 2 neuropathies and isolated optic atrophy.

European journal of neurology

Cipriani S,Guerrero-Valero M,Tozza S,Zhao E,Vollmer V,Beijer D,Danzi M,Rivellini C,Lazarevic D,Pipitone GB,Grosz BR,Lamperti C,Marzoli SB,Carrera P,Devoto M,Pisciotta C,Pareyson D,Kennerson M,Previtali SC,Zuchner S,Scherer SS,Manganelli F,Bähler M,Bolino A

Wed Feb 01 00:00:00 EST 2023

Mutations in MYO9B are associated with Charcot-Marie-Tooth disease type 2 neuropathies and isolated optic atrophy.

European journal of neurology

Wed Feb 01 00:00:00 EST 2023

Mutations in MYO9B are associated with Charcot-Marie-Tooth disease type 2 neuropathies and isolated optic atrophy.

European journal of neurology

Wed Feb 01 00:00:00 EST 2023

Mutations in MYO9B are associated with Charcot-Marie-Tooth disease type 2 neuropathies and isolated optic atrophy.

European journal of neurology

Wed Feb 01 00:00:00 EST 2023

TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant Pleural Effusion by Regulating T_H1/T_H17 Cell Response.

Journal of immunology (Baltimore, Md. : 1950)

Yi FS,Zhang X,Zhai K,Huang ZY,Wu XZ,Wu MT,Shi XY,Pei XB,Dong SF,Wang W,Yang Y,Du J,Luo ZT,Shi HZ

Sun Nov 15 00:00:00 EST 2020

The RhoA regulators Myo9b and GEF-H1 are targets of cyclic nucleotide-dependent kinases in platelets.

Journal of thrombosis and haemostasis : JTH

Comer S,Nagy Z,Bolado A,von Kriegsheim A,Gambaryan S,Walter U,Pagel O,Zahedi RP,Jurk K,Smolenski A

Sun Nov 01 00:00:00 EDT 2020

The RhoGAP Myo9b Promotes Bone Growth by Mediating Osteoblastic Responsiveness to IGF-1.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

McMichael BK,Jeong YH,Auerbach JA,Han CM,Sedlar R,Shettigar V,Bähler M,Agarwal S,Kim DG,Lee BS

Sun Oct 01 00:00:00 EDT 2017

The RhoGAP Myo9b Promotes Bone Growth by Mediating Osteoblastic Responsiveness to IGF-1.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

McMichael BK,Jeong YH,Auerbach JA,Han CM,Sedlar R,Shettigar V,Bähler M,Agarwal S,Kim DG,Lee BS

Sun Oct 01 00:00:00 EDT 2017

Noncanonical Myo9b-RhoGAP Accelerates RhoA GTP Hydrolysis by a Dual-Arginine-Finger Mechanism.

Journal of molecular biology

Yi F,Kong R,Ren J,Zhu L,Lou J,Wu JY,Feng W

Sun Jul 31 00:00:00 EDT 2016

Noncanonical Myo9b-RhoGAP Accelerates RhoA GTP Hydrolysis by a Dual-Arginine-Finger Mechanism.

Journal of molecular biology

Yi F,Kong R,Ren J,Zhu L,Lou J,Wu JY,Feng W

Sun Jul 31 00:00:00 EDT 2016

Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.

Cell reports

Makowska KA,Hughes RE,White KJ,Wells CM,Peckham M

Tue Dec 15 00:00:00 EST 2015

Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.

Cell reports

Makowska KA,Hughes RE,White KJ,Wells CM,Peckham M

Tue Dec 15 00:00:00 EST 2015

Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.

Cell reports

Makowska KA,Hughes RE,White KJ,Wells CM,Peckham M

Tue Dec 15 00:00:00 EST 2015

Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.

Cell reports

Makowska KA,Hughes RE,White KJ,Wells CM,Peckham M

Tue Dec 15 00:00:00 EST 2015

The RhoGAP activity of myosin IXB is critical for osteoclast podosome patterning, motility, and resorptive capacity.

PloS one

McMichael BK,Scherer KF,Franklin NC,Lee BS

Mon Sep 29 00:00:00 EDT 2014

The RhoGAP activity of myosin IXB is critical for osteoclast podosome patterning, motility, and resorptive capacity.

PloS one

McMichael BK,Scherer KF,Franklin NC,Lee BS

Mon Sep 29 00:00:00 EDT 2014

The RhoGAP activity of myosin IXB is critical for osteoclast podosome patterning, motility, and resorptive capacity.

PloS one

McMichael BK,Scherer KF,Franklin NC,Lee BS

Mon Sep 29 00:00:00 EDT 2014

The RhoGAP activity of myosin IXB is critical for osteoclast podosome patterning, motility, and resorptive capacity.

PloS one

McMichael BK,Scherer KF,Franklin NC,Lee BS

Mon Sep 29 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: celiac disease 4; myosin; myosin-IXb; myr 5; Unconventional myosin from rat 3; unconventional myosin IXb; Unconventional myosin-9b; Unconventional myosin-IXb

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Gene Aliases: CELIAC4; MYO9B; MYR5

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UniProt ID: (Human) Q13459

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Entrez Gene ID: (Human) 4650, (Mouse) 17925, (Rat) 25486

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Function(s)

microfilament motor activity actin binding GTPase activator activity protein binding calmodulin binding ATP binding zinc ion binding ATPase activity Rho GTPase binding actin-dependent ATPase activity protein homodimerization activity ATP-dependent protein binding ADP binding Roundabout binding actin filament binding nucleotide binding motor activity metal ion binding

Process(es)

Rho protein signal transduction actin filament-based movement ARF protein signal transduction regulation of Rho protein signal transduction Roundabout signaling pathway positive regulation of GTPase activity regulation of small GTPase mediated signal transduction monocyte chemotaxis signal transduction metabolic process establishment of cell polarity actin-myosin filament sliding intracellular signal transduction macrophage chemotaxis lamellipodium morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MYO9B Polyclonal Antibody

MYO9B Antibody (12432-1-AP) in IHC (P)

Product Details

12432-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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