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MYOC Antibody (PA5-90794) in WB
Western blot analysis of MYOC in extracts of various cell lines. Samples were incubated with MYOC Polyclonal antibody (Product # PA5-90794) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-90794
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 245-504 of human MYOC (NP_0002521)
Conjugate
Form
Liquid
Concentration
0.46 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806240
Product Specific Information
Immunogen sequence: CGELVWVGEP LTLRTAETIT GKYGVWMRDP KPTYPYTQET TWRIDTVGTD VRQVFEYDLI SQFMQGYPSK VHILPRPLES TGAVVYSGSL YFQGAESRTV IRYELNTETV KAEKEIPGAG YHGQFPYSWG GYTDIDLAVD EAGLWVIYST DEAKGAIVLS KLNPENLELE QTWETNIRKQ SVANAFIICG TLYTVSSYTS ADATVNFAYD TGTGISKTLT IPFKNRYKYS SMIDYNPLEK KLFAWDNLNM VTYDIKLSKM; Positive Samples: Raji, U-937, BT-474, Mouse brain; Cellular Location: Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Mitochondrion, Mitochondrion inner membrane, Mitochondrion intermembrane space, Mitochondrion outer membrane, Rough endoplasmic reticulum, cilium, exosome, extracellular matrix, extracellular space
Target Information
Myocilin is an extracellular protein expressed in the eye, including the retina, trabecular meshwork and ciliary body. Myocilin can form homomultimers in vivo and can also associate with components of the ECM via interactions with the Hep II domain of FibroPVRL1. In addition, myocilin interacts with myosin regulatory light chain, a component of the myosin motor protein complex. This interaction implies a role for myocilin in the actomyosin system, linking myocilin to the functional status of the trabecular meshwork (TM), which is responsible for controlling the intraocular pressure (IOP). Alterations in functions of the TM may lead to IOP elevation and development of glaucoma, a major cause of blindness. Myocilin is encoded by MYOC (also designated TIGR), a gene that maps to the GLC1A locus on chromosome 1q24. 3 and is susceptible to mutations. Mutations in the MYOC gene are specifically linked with primary open angle glaucoma (POAG), a blinding disease characterized by progressive loss of retinal ganglion cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: juvenile-onset open-angle glaucoma 1; mutated trabecular meshwork-induced glucocorticoid response protein; Myocilin; Myocilin 55 kDa subunit; myocilin trabecular meshwork inducible glucocorticoid response; myocilin trabecular meshwork inducible glucocorticoid response protein; myocilin, trabecular meshwork inducible glucocorticoid response; trabecular meshwork inducible glucocorticoid response protein; Trabecular meshwork-induced glucocorticoid response protein
Gene Aliases: AI957332; GLC1A; GPOA; JOAG; JOAG1; MYOC; TIGR
UniProt ID: (Human) Q99972, (Mouse) O70624
Entrez Gene ID: (Human) 4653, (Mouse) 17926
osteoblast differentiation
negative regulation of cell-matrix adhesion
positive regulation of phosphatidylinositol 3-kinase signaling
skeletal muscle hypertrophy
myelination in peripheral nervous system
positive regulation of cell migration
neuron projection development
negative regulation of Rho protein signal transduction
non-canonical Wnt signaling pathway via JNK cascade
ERBB2-ERBB3 signaling pathway
regulation of MAPK cascade
clustering of voltage-gated sodium channels
positive regulation of stress fiber assembly
negative regulation of stress fiber assembly
positive regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
positive regulation of mitochondrial depolarization
bone development
positive regulation of substrate adhesion-dependent cell spreading
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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