Invitrogen

MYOD Polyclonal Antibody

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MYOD Polyclonal Antibody

Product Details

PA5-110005

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 226-300 of human MyoD1 (NP_0024692)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.51 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2855416

Product Specific Information

Immunogen sequence: YEGAYYNEAP SEPRPGKSAA VSSLDCLSSI VERISTESPA APALLLADVP SESPPRRQEA AAPSEGESSG DPTQS

Target Information

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bHLHc1; Class C basic helix-loop-helix protein 1; Myf-3; Myoblast determination protein 1; Myogenic factor 3

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Gene Aliases: AI503393; BHLHC1; MYF3; MYOD; Myod-1; MYOD1; PUM

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UniProt ID: (Human) P15172, (Mouse) P10085

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Entrez Gene ID: (Human) 4654, (Mouse) 17927

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity protein binding transcription factor binding chromatin DNA binding ubiquitin protein ligase binding nuclear hormone receptor binding protein heterodimerization activity E-box binding DNA binding transcription factor activity, sequence-specific DNA binding enzyme binding sequence-specific DNA binding protein dimerization activity basic helix-loop-helix transcription factor
Process(es)
regulation of alternative mRNA splicing, via spliceosome regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein phosphorylation muscle organ development myoblast fate determination skeletal muscle tissue development myoblast fusion cellular response to starvation myotube cell development myotube differentiation involved in skeletal muscle regeneration skeletal muscle cell differentiation muscle cell fate commitment positive regulation of skeletal muscle tissue regeneration regulation of RNA splicing skeletal muscle fiber adaptation histone H3 acetylation histone H4 acetylation positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter skeletal muscle fiber development positive regulation of skeletal muscle fiber development positive regulation of muscle cell differentiation cellular response to glucocorticoid stimulus cellular response to estradiol stimulus cellular response to oxygen levels positive regulation of myoblast fusion negative regulation of myoblast proliferation transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development myotube differentiation cell differentiation skeletal muscle tissue regeneration myoblast differentiation positive regulation of transcription, DNA-templated striated muscle cell differentiation cardiac muscle cell differentiation
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