Invitrogen

MYOD Polyclonal Antibody

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Antibody Testing Data (2)
Published Figures (12)

FIGURE: 1 / 14

MYOD Antibody (PA5-23078) in WB

Western blot was performed using Anti-MYOD Polyclonal Antibody (Product # PA5-23078) and a 40 kDa band corresponding to MYOD was observed across all the cell lines tested except SK-N-AS which is reported to be low expressing cell line and was increased in C2C12 upon differentiation to myotubes along with an uncharacterized band at ~25 kDa. Modified whole cell extracts (1% SDS) (30 µg lysate) of C2C12 (Lane 1), C2C12 differentiated to myotubes (3 days) (Lane 2), C2C12 differentiated to myotubes (7 days) (Lane 3), RD (Lane 4), EB-2 (Lane 5) ... View More

MYOD Antibody in Immunocytochemistry (ICC/IF)

MYOD Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

MYOD Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-23078

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to an N-terminal region of the human MyoD1 protein (within residues 1-150).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152869

Product Specific Information

The target sequence has 90% sequence homology with mouse.

Target Information

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An in vitro Mechanical Damage Model of Isolated Myofibers in a Floating Culture Condition.

Bio-protocol

Tsuchiya Y,Ono Y

PA5-23078 was used in Immunocytochemistry to describe a protocol for the mechanical damage of myofibers and co-culture of intact healthy myofibers with damaged myofibers in a floating condition.

Wed Jan 05 00:00:00 EST 2022

Combination of cell signaling molecules can facilitate MYOD1-mediated myogenic transdifferentiation of pig fibroblasts.

Journal of animal science and biotechnology

Jeong J,Choi KH,Kim SH,Lee DK,Oh JN,Lee M,Choe GC,Lee CK

Published figure using MYOD polyclonal antibody (Product # PA5-23078) in Immunocytochemistry

Thu May 13 00:00:00 EDT 2021

Stimulation of Non-canonical NF-κB Through Lymphotoxin-β-Receptor Impairs Myogenic Differentiation and Regeneration of Skeletal Muscle.

Frontiers in cell and developmental biology

Schmidt M,Weidemann A,Poser C,Bigot A,von Maltzahn J

Published figure using MYOD polyclonal antibody (Product # PA5-23078) in Immunohistochemistry

Sat Oct 23 00:00:00 EDT 2021

MiR-455-5p monitors myotube morphogenesis by targeting mylip.

Journal of cellular biochemistry

Dong YH,Zhou CJ,Zhang MY,Tao J,Zhang XM,An L,Zhang J,Yang J,Liu DJ,Cang M

PA5-23078 was used in Western Blotting to uncover novel miR-455-5p targets and establish a functional link between Mylip and myotube morphogenesis.

Thu Apr 01 00:00:00 EDT 2021

MiR-455-5p monitors myotube morphogenesis by targeting mylip.

Journal of cellular biochemistry

Dong YH,Zhou CJ,Zhang MY,Tao J,Zhang XM,An L,Zhang J,Yang J,Liu DJ,Cang M

PA5-23078 was used in Western Blotting to uncover novel miR-455-5p targets and establish a functional link between Mylip and myotube morphogenesis.

Thu Apr 01 00:00:00 EDT 2021

MiR-455-5p monitors myotube morphogenesis by targeting mylip.

Journal of cellular biochemistry

Dong YH,Zhou CJ,Zhang MY,Tao J,Zhang XM,An L,Zhang J,Yang J,Liu DJ,Cang M

PA5-23078 was used in Western Blotting to uncover novel miR-455-5p targets and establish a functional link between Mylip and myotube morphogenesis.

Thu Apr 01 00:00:00 EDT 2021

FOXP1 Interacts with MyoD to Repress its Transcription and Myoblast Conversion.

Journal of cellular signaling

Wright WE,Li C,Zheng CX,Tucker HO

Published figure using MYOD polyclonal antibody (Product # PA5-23078) in Western Blot

Thu Feb 11 00:00:00 EST 2021

The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation.

Scientific reports

Norizadeh Abbariki T,Gonda Z,Kemler D,Urbanek P,Wagner T,Litfin M,Wang ZQ,Herrlich P,Kassel O

Published figure using MYOD polyclonal antibody (Product # PA5-23078) in Immunocytochemistry

Fri Jun 18 00:00:00 EDT 2021

The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation.

Scientific reports

Norizadeh Abbariki T,Gonda Z,Kemler D,Urbanek P,Wagner T,Litfin M,Wang ZQ,Herrlich P,Kassel O

Published figure using MYOD polyclonal antibody (Product # PA5-23078) in Immunocytochemistry

Fri Jun 18 00:00:00 EDT 2021

Wnt7a Counteracts Cancer Cachexia.

Molecular therapy oncolytics

Schmidt M,Poser C,von Maltzahn J

Published figure using MYOD polyclonal antibody (Product # PA5-23078) in Immunocytochemistry

Fri Mar 27 00:00:00 EDT 2020

MBNL1 reverses the proliferation defect of skeletal muscle satellite cells in myotonic dystrophy type 1 by inhibiting autophagy via the mTOR pathway.

Cell death & disease

Song KY,Guo XM,Wang HQ,Zhang L,Huang SY,Huo YC,Zhang G,Feng JZ,Zhang RR,Ma Y,Hu QZ,Qin XY

PA5-23078 was used in Immunocytochemistry-immunoflourescence to illustrate the proliferation defect of skeletal muscle satellite cells (SSCs) differentiated from DM1 patient-derived iPSCs, and suggest that genome modification can eliminate nuclear RNA foci and enhance the proliferation of DM1 SSCs.

Sat Jul 18 00:00:00 EDT 2020

MBNL1 reverses the proliferation defect of skeletal muscle satellite cells in myotonic dystrophy type 1 by inhibiting autophagy via the mTOR pathway.

Cell death & disease

Song KY,Guo XM,Wang HQ,Zhang L,Huang SY,Huo YC,Zhang G,Feng JZ,Zhang RR,Ma Y,Hu QZ,Qin XY

Sat Jul 18 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: bHLHc1; Class C basic helix-loop-helix protein 1; Myf-3; Myoblast determination protein 1; Myogenic factor 3

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Gene Aliases: AI503393; BHLHC1; MYF3; MYOD; Myod-1; MYOD1; PUM

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UniProt ID: (Human) P15172, (Mouse) P10085

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Entrez Gene ID: (Human) 4654, (Mouse) 17927

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity protein binding transcription factor binding chromatin DNA binding ubiquitin protein ligase binding nuclear hormone receptor binding protein heterodimerization activity E-box binding DNA binding transcription factor activity, sequence-specific DNA binding enzyme binding sequence-specific DNA binding protein dimerization activity basic helix-loop-helix transcription factor

Process(es)

regulation of alternative mRNA splicing, via spliceosome regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein phosphorylation muscle organ development myoblast fate determination skeletal muscle tissue development myoblast fusion cellular response to starvation myotube cell development myotube differentiation involved in skeletal muscle regeneration skeletal muscle cell differentiation muscle cell fate commitment positive regulation of skeletal muscle tissue regeneration regulation of RNA splicing skeletal muscle fiber adaptation histone H3 acetylation histone H4 acetylation positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter skeletal muscle fiber development positive regulation of skeletal muscle fiber development positive regulation of muscle cell differentiation cellular response to glucocorticoid stimulus cellular response to estradiol stimulus cellular response to oxygen levels positive regulation of myoblast fusion negative regulation of myoblast proliferation transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development myotube differentiation cell differentiation skeletal muscle tissue regeneration myoblast differentiation positive regulation of transcription, DNA-templated striated muscle cell differentiation cardiac muscle cell differentiation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MYOD Polyclonal Antibody

MYOD Antibody (PA5-23078) in WB

Product Details

PA5-23078

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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