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MYOM2 Antibody (PA5-120745) in WB
Western blot analysis of MYOM2 in extracts of various cell lines. Samples were incubated with MYOM2 Polyclonal antibody (Product # PA5-120745) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-120745
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human MYOM2 (NP_003961.3).
Conjugate
Form
Liquid
Concentration
3.78 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914317
Product Specific Information
Positive test controls include: Mouse heart, Mouse skeletal muscle, Rat heart, Rat skeletal muscle.^M
^M
Immunogen sequence: MSLVTVPFYQ KRHRHFDQSY RNIQTRYLLD EYASKKRAST QASSQKSLSQ RSSSQRASSQ TSLGGTICRV CAKRVSTQED EEQENRSRYQ SLVAAYGEAK RQRFLSELAH LEEDVHLARS QARDKLDKYA IQQMMEDKLA WERHTFEERI SRAPEILVRL RSHTVWERMS VKLCFTVQGF PTPVVQWYKD GSLICQAAEP
Target Information
The giant protein titin, together with its associated proteins, interconnects the major structure of sarcomeres, the M bands and Z discs. The C-terminal end of the titin string extends into the M line, where it binds tightly to M-band constituents of apparent molecular masses of 190 kD and 165 kD. The predicted MYOM2 protein contains 1,465 amino acids. Like MYOM1, MYOM2 has a unique N-terminal domain followed by 12 repeat domains with strong homology to either fibronectin type III or immunoglobulin C2 domains. Protein sequence comparisons suggested that the MYOM2 protein and bovine M protein are identical. [provided by RefSeq, Jul 2008].
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 165 kDa connectin-associated protein; 165 kDa titin-associated protein; M-band protein; M-protein; myomesin (M-protein) 2, 165kDa; Myomesin family member 2; Myomesin-2; skeletal muscle 165kD protein; skeletal muscle 165kDa protein; titin-associated protein, 165 kD
Gene Aliases: AW146149; MYOM2; TTNAP
UniProt ID: (Human) P54296
Entrez Gene ID: (Human) 9172, (Mouse) 17930, (Rat) 306616
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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