Invitrogen

MYPT1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

5 Published Figures

2 References

View all (22) MYPT1 antibodies

Datasheet

Datasheet

Product Image Gallery

MYPT1 Antibody — Published figure using MYPT1 polyclonal antibody (Product # PA5-17164)

MYPT1 Antibody in Western Blot (WB) — Knockdown of MYPT1 was achieved by transfecting HeLa cells with MYPT1 specific siRNAs (Silencer® select Product # s9237, s9235). Western blot analysis (Fig. a) was performed using whole cell extracts from the MYPT1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-MYPT1 Polyclonal Antibody (Product # PA5-17164, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MYPT1.

MYPT1 Antibody in KnockDown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with MYPT1 siRNA and reduction of signal was observed in Western Blot using MYPT1 Polyclonal Antibody (Product # PA5-17164). {KD}

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:1000

Published Applications

Western Blot (WB)

See 2 publications below

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Pig, Rat, Zebrafish

Published species

Human, Pig

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide correspondent to amino-terminal residues of human MYPT1

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

RRID

AB_10978517

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other family members.

Target Information

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 130 kDa myosin-binding subunit of smooth muscle myosin phophatase; 130 kDa myosin-binding subunit of smooth muscle myosin phosphatase; 133kDa myosin-binding subunit of smooth muscle myosin phosphatase (M133); MBSP; myosin binding subunit; Myosin phosphatase target subunit 1; myosin phosphatase, target subunit 1; Myosin phosphatase-targeting subunit 1; myosin-binding subunit of myosin phosphatase; mypt1; PP-1M; PP1M subunit M110; Protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase 1, regulatory subunit 12A; Protein phosphatase myosin-binding subunit; Protein phosphatase subunit 1M; Serine/threonine protein phosphatase PP1 smooth muscle regulatory subunit M110; smooth muscle myosin phosphatase myosin-binding subunit; unm p82emcf

Gene Aliases: 1200015F06Rik; 5730577I22Rik; AA792106; AV099298; BOS_4870; D10Ertd625e; I79_016312; M110; M130; MBS; MBSP; MYPT1; PPP1R12A; RCJMB04_22k13; si:dkey-28j4.1; unm_p82emcf; zgc:110448

UniProt ID: (Chicken) Q90623, (Human) O14974, (Rat) Q10728, (Mouse) Q9DBR7, (Zebrafish) Q6DRG7

Entrez Gene ID: (Pig) 397190, (Chicken) 396020, (Bovine) 538606, (Dog) 475411, (Human) 4659, (Rat) 116670, (Mouse) 17931, (Chinese hamster) 100754718, (Zebrafish) 445393

Molecular Function: enzyme modulator phosphatase modulator