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Invitrogen
MYPT1 Polyclonal Antibody
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FIGURE: 1 / 11
MYPT1 Antibody (PA5-17164) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-2 OS (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), HEK293 (Lane 4) and U-87 MG (Lane 5). The blot was probed with Anti-MYPT1 Polyclonal Antibody (Product # PA5-17164, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 130kDa band corresponding to MYPT1 was seen in all cell lines tested. Additional uncharacterized bands were also ob... View More
Product Details
PA5-17164
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse,
Pig
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide correspondent to amino-terminal residues of human MYPT1
Conjugate
Form
Liquid
Concentration
5 µg/mL
Amount
0.5 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other family members.
Target Information
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP. RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MBSP; myosin binding subunit; Myosin phosphatase target subunit 1; myosin phosphatase, target subunit 1; Myosin phosphatase-targeting subunit 1; PP-1M; Protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase 1, regulatory subunit 12A; Protein phosphatase myosin-binding subunit; Protein phosphatase subunit 1M; Serine/threonine protein phosphatase PP1 smooth muscle regulatory subunit M110; smooth muscle myosin phosphatase myosin-binding subunit; unnamed protein product
Gene Aliases: 1200015F06Rik; 5730577I22Rik; AA792106; AV099298; D10Ertd625e; GUBS; I79_016312; M110; M130; MBS; MBSP; MYPT1; PPP1R12A
UniProt ID: (Human) O14974, (Rat) Q10728, (Mouse) Q9DBR7
Entrez Gene ID: (Human) 4659, (Rat) 116670, (Mouse) 17931, (Chinese hamster) 100754718
mitotic cell cycle
protein dephosphorylation
centrosome cycle
signal transduction
regulation of cell adhesion
negative regulation of catalytic activity
positive regulation of transcription from RNA polymerase II promoter
regulation of nucleocytoplasmic transport
neuron projection morphogenesis
cellular response to xenobiotic stimulus
regulation of establishment of endothelial barrier
mitotic nuclear division
regulation of myosin-light-chain-phosphatase activity
centrosome organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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