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      MYPT1 Polyclonal Antibody

      See 8 other MYPT1 antibodies ›
      MYPT1 Polyclonal Antibody for Western Blot

      Catalog#: PA5-17164 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      PA5-17164 100 µL
      275.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Rabbit Secondary Antibodies (264)
      Alexa Fluor® Anti-Rabbit Secondary Antibodies (69)
      HRP Anti-Rabbit Secondary Antibodies (30)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Bovine, Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Pig, Rat, Zebrafish
      Published species reactivity Pig, Human
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen Synthetic peptide correspondent to amino-terminal residues of human MYPT1
      Conjugate Unconjugated
      Form Liquid
      Purification Antigen affinity chromatography
      Storage buffer 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol
      Contains no preservative
      Storage Conditions -20°C
      Tested Applications Dilution *
      Western Blot (WB) 1:1000

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Western Blot (WB) See 2 publications below

      Product Specific Information

      It is not recommended to aliquot this antibody.

      This antibody is not cross-reactive with other family members.

      Background/Target Information

      Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Western Blot

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Pig 1:40000 Heterogeneity in relaxation of different sized porcine coronary arteries to nitrovasodilators: role of PKG and MYPT1.
      PA5-17164 was used in western blot to investigate the role of cGMP-dependent protein kinase and myosin phosphatase target subunit 1 in the response of large coronary arteries to nitrovasodilators       		Ying L,Xu X,Liu J,Dou D,Yu X,Ye L,He Q,Gao Y Pflugers Archiv : European journal of physiology (463:257) 2012
      Human 1:40000 Increased degradation of MYPT1 contributes to the development of tolerance to nitric oxide in porcine pulmonary artery.
      PA5-17164 was used in western blot to characterize MYPT1-mediated NO tolerance in the pulmonary artery       		Ma H,He Q,Dou D,Zheng X,Ying L,Wu Y,Raj JU,Gao Y American journal of physiology. Lung cellular and molecular physiology (299:L117) 2010

      Bioinformatics

      Protein Aliases:

      130 kDa myosin-binding subunit of smooth muscle myosin phophatase; 130 kDa myosin-binding subunit of smooth muscle myosin phosphatase; 133kDa myosin-binding subunit of smooth muscle myosin phosphatase (M133); MBSP; myosin binding subunit; Myosin phosphatase target subunit 1; myosin phosphatase targeting subunit 1; myosin phosphatase, target subunit 1; Myosin phosphatase-targeting subunit 1; myosin-binding subunit of myosin phosphatase; mypt1; PP-1M; PP1M subunit M110; Protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase 1, regulatory subunit 12A; Protein phosphatase myosin-binding subunit; Protein phosphatase subunit 1M; Serine/threonine protein phosphatase PP1 smooth muscle regulatory subunit M110; smooth muscle myosin phosphatase myosin-binding subunit

      Gene Aliases:

      1200015F06Rik; 5730577I22Rik; AA792106; AV099298; BOS_4870; D10Ertd625e; DKEY-28J4.1; I79_016312; M110; M130; MBS; MBSP; MYPT1; PPP1R12A; RCJMB04_22k13; si:dkey-28j4.1; zgc:110448

      UniProt ID:

      (Chicken) Q90623, (Human) O14974, (Rat) Q10728, (Mouse) Q9DBR7, (Zebrafish) Q6DRG7

      Entrez Gene ID:

      (Pig) 397190, (Chicken) 396020, (Bovine) 538606, (Dog) 475411, (Human) 4659, (Rat) 116670, (Mouse) 17931, (Chinese hamster) 100754718, (Zebrafish) 445393

      Molecular Function:

      enzyme modulator phosphatase modulator

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

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