Invitrogen

MYPT1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

3 References

View all (34) MYPT1 antibodies

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Datasheet

Protocols

Questions & Answers

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MYPT1 Antibody in Western Blot (WB) — Knockdown of MYPT1 was achieved by transfecting HeLa cells with MYPT1 specific siRNAs (Silencer® select Product # s9237, s9235). Western blot analysis (Fig. a) was performed using whole cell extracts from the MYPT1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-MYPT1 Polyclonal Antibody (Product # PA5-17164, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MYPT1.

MYPT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with MYPT1 siRNA and reduction of signal was observed in Western Blot using MYPT1 Polyclonal Antibody (Product # PA5-17164). {KD}

Product Details

PA5-17164

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 3 publications 3 publications

Product Specifications
Species Reactivity	Bovine, Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Pig, Rat, Zebrafish
Published species	Human, Pig
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide correspondent to amino-terminal residues of human MYPT1 View antigen
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	no preservative
Storage conditions	-20°C
RRID	AB_10978517

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other family members.

Target Information

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP. RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The IGF1 Receptor Is Involved in Follicle-Stimulating Hormone Signaling in Porcine Neonatal Sertoli Cells.

Journal of clinical medicine

Cannarella R,Arato I,Condorelli RA,Luca G,Barbagallo F,Alamo A,Bellucci C,Lilli C,La Vignera S,Calafiore R,Mancuso F,Calogero AE

PA5-17164 was used in Western Blotting to evaluate the role of the IGF1 Receptor in Follicle-Hormone Signaling in Sertoli Cells.

Sat Apr 27 00:00:00 EDT 2019

Heterogeneity in relaxation of different sized porcine coronary arteries to nitrovasodilators: role of PKG and MYPT1.

Pflugers Archiv : European journal of physiology

Ying L,Xu X,Liu J,Dou D,Yu X,Ye L,He Q,Gao Y

PA5-17164 was used in western blot to investigate the role of cGMP-dependent protein kinase and myosin phosphatase target subunit 1 in the response of large coronary arteries to nitrovasodilators

Wed Feb 01 00:00:00 EST 2012

Increased degradation of MYPT1 contributes to the development of tolerance to nitric oxide in porcine pulmonary artery.

American journal of physiology. Lung cellular and molecular physiology

Ma H,He Q,Dou D,Zheng X,Ying L,Wu Y,Raj JU,Gao Y

PA5-17164 was used in western blot to characterize MYPT1-mediated NO tolerance in the pulmonary artery

Thu Jul 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: 130 kDa myosin-binding subunit of smooth muscle myosin phophatase; 130 kDa myosin-binding subunit of smooth muscle myosin phosphatase; 130 kDa regulatory subunit of myosin phosphatase; 133kDa myosin-binding subunit of smooth muscle myosin phosphatase (M133); MBSP; myosin binding subunit; Myosin phosphatase target subunit 1; myosin phosphatase, target subunit 1; Myosin phosphatase-targeting subunit 1; myosin-binding subunit of myosin phosphatase; mypt1; PP-1M; PP1M subunit M110; Protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase 1, regulatory subunit 12A; protein phosphatase 1M 110 kda regulatory subunit; Protein phosphatase myosin-binding subunit; Protein phosphatase subunit 1M; Serine/threonine protein phosphatase PP1 smooth muscle regulatory subunit M110; smooth muscle myosin phosphatase myosin-binding subunit; unm p82emcf

Gene Aliases: 1200015F06Rik; 5730577I22Rik; AA792106; AV099298; D10Ertd625e; I79_016312; M110; M130; MBS; MBSP; MYPT1; PPP1R12A; si:dkey-28j4.1; unm_p82emcf; zgc:110448

UniProt ID: (Chicken) Q90623, (Human) O14974, (Rat) Q10728, (Mouse) Q9DBR7, (Zebrafish) Q6DRG7

Entrez Gene ID: (Pig) 397190, (Chicken) 396020, (Bovine) 538606, (Dog) 475411, (Human) 4659, (Rat) 116670, (Mouse) 17931, (Chinese hamster) 100754718, (Zebrafish) 445393

Molecular Function: phosphatase modulator protein-binding activity modulator