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Invitrogen
MYPT1 Polyclonal Antibody
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FIGURE: 1 / 1
MYPT1 Antibody (PA5-78068) in ICC/IF
MYPT1 Polyclonal Antibody detects MYPT1 protein at cytoplasm by immunofluorescent analysis. Sample: RD cells were fixed in ice-cold MeOH for 5 min. Green: MYPT1 protein stained by MYPT1 Polyclonal Antibody (Product # PA5-78068) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Product Details
PA5-78068
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human MYPT1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Predicted Reactivity: Mouse (91%), Rat (93%), Chicken (85%), Rhesus Monkey (99%), Bovine (96%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP. RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: myosin binding subunit; Myosin phosphatase target subunit 1; myosin phosphatase, target subunit 1; Myosin phosphatase-targeting subunit 1; Protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase 1, regulatory subunit 12A; Protein phosphatase myosin-binding subunit
Gene Aliases: M130; MBS; MYPT1; PPP1R12A
UniProt ID: (Human) Q8NFR6
Entrez Gene ID: (Human) 4659
G2/M transition of mitotic cell cycle
protein dephosphorylation
mitotic nuclear division
signal transduction
regulation of cell adhesion
regulation of myosin-light-chain-phosphatase activity
positive regulation of myosin-light-chain-phosphatase activity
cellular response to drug
negative regulation of catalytic activity
positive regulation of transcription from RNA polymerase II promoter
regulation of nucleocytoplasmic transport
centrosome organization
regulation of establishment of endothelial barrier
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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