Invitrogen

MYT1L Polyclonal Antibody

View all (5) MYT1L antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

MYT1L Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse cerebellum tissue using MYT1L Polyclonal Antibody (Product # PA5-119925). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the MYT1L antibody at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

MYT1L Antibody in Western Blot (WB) — Western blot analysis of MyT1L on SHSY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. MYT1L Polyclonal Antibody (Product # PA5-119925) at 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

MYT1L Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of MYT1L in SH-SY5Y cells using MYT1L Polyclonal Antibody (Product # PA5-119925) at 1 µg/mL (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at 4°C for 1 hour, the cells were stained with a Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution for 30 minutes at 4°C (dark incubation). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Product Details

PA5-119925

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:500	-
Flow Cytometry (Flow)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein within human MyT1L aa 200-400/1186 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2913497

Product Specific Information

Positive Control: SHSY5Y cell lysates, mouse cerebellum tissue, mouse brain tissue, SH-SY5Y.

Subcellular Location: Chromosome, Nucleus.

Target Information

MYT1L, myelin transcription factor 1 family, to which the myelin transcription factor 1-like protein (MYT1L) belongs, is expressed primarily in the developing central nervous system and recruits histone deacetylase (HDAC) to regulate neural transcription. Both MYT1L and the related protein MYT1 interact with SIN3B, a protein that mediates transcriptional repression by binding to HDACs, suggesting that the MYT1 family favor the silencing of genes during neural development. Recent studies suggest that polymorphisms of MYT1L may be associated with schizophrenia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Myelin transcription factor 1-like protein; MyT1-L; Neural zinc finger factor 1; neural zinc finger protein NZF-1; neural zinc finger transcription factor 1; NZF-1; postmeiotic neural gene 1; Postmitotic neural gene 1 protein; Zinc finger protein Png-1

Gene Aliases: 2900046C06Rik; 2900093J19Rik; C630034G21Rik; KIAA1106; mKIAA1106; MRD39; MYT1L; NZF1; Nztf1; Pmng1; Png-1; Png1; ZC2H2C2; ZC2HC4B

UniProt ID: (Human) Q9UL68, (Mouse) P97500

Entrez Gene ID: (Human) 23040, (Mouse) 17933

Molecular Function: zinc finger transcription factor