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Abnova
Mapk8 Recombinant Rabbit Monoclonal Antibody
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FIGURE: 1 / 2
Mapk8 Antibody (RAB02516) in ICC/IF
Immunofluorescent analysis of HeLa cells with JNK1 recombinant monoclonal antibody (Product # RAB02516). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Product Details
RAB02516
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
50mM tris glycine, pH 7.4, with 0.05% BSA, 150mM NaCl, 50% glycerol
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
MAPK8 (JNK1) is activated by the presence of misfolded proteins in the endoplasmic reticulum. It is also a crucial mediator of insulin resistance and obesity. It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-Jun N-terminal kinase 1; FLJ12099; FLJ33785; JNK1 beta1 protein kinase; JNK3; JNK3A; MAP kinase 10; MAP kinase 8; MAPK 10; MAPK 8; MAPK10; MGC50974; mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; MK10; OTTHUMP00000019552; p493F12; p54 gamma; p54bSAPK; Phospho-JNK; PRKM10; protein kinase JNK1; protein kinase mitogen-activated 8; SAPK gamma; Stress-activated protein kinase JNK1
Gene Aliases: AI849689; JNK; JNK1; Mapk8; Prkm8; SAPK1
UniProt ID: (Rat) P49185, (Mouse) Q91Y86
Entrez Gene ID: (Rat) 116554, (Mouse) 26419
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
JUN kinase activity
MAP kinase activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
enzyme binding
histone deacetylase regulator activity
histone deacetylase binding
non-receptor serine/threonine protein kinase
ossification
regulation of transcription, DNA-templated
protein phosphorylation
inflammatory response
response to osmotic stress
signal transduction
JNK cascade
JUN phosphorylation
response to heat
response to UV
regulation of gene expression
positive regulation of gene expression
phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
positive regulation of cell migration
regulation of histone deacetylation
positive regulation of microtubule polymerization
neuron projection development
positive regulation of cyclase activity
negative regulation of protein binding
regulation of protein localization
response to hydrogen peroxide
regulation of circadian rhythm
negative regulation of apoptotic process
positive regulation of DNA replication
response to cadmium ion
determination of dorsal identity
rhythmic process
positive regulation of protein metabolic process
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to nitric oxide
positive regulation of deacetylase activity
type B pancreatic cell apoptotic process
neuronal stem cell population maintenance
programmed necrotic cell death
positive regulation of determination of dorsal identity
positive regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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