|Western Blot (WB)||2.0 µg/ml|
|Western Blot (WB)||See 3 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Dog , Rabbit , Rat , Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: L(90) Y H T E D T R D Q N Q Q C(103)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-924 detects large conductance, calcium-activated potassium channel beta (Maxi K+ Beta) from human, mouse, and rat samples.
PA1-924 has been successfully used in western blot and immunofluorescence. By western blot, this antibody detects an ~28kDa protein representing Maxi K+ beta from mouse myometrial smooth muscle cell extracts. The molecular weight detected varies due to glycosylation, multimerization and binding to actin.
Immunizing peptide corresponds to amino acid residues 90-103 from human Maxi K+ beta. This sequence is completely conserved across many species. PA1-924 immunizing peptide (Cat. # PEP-119) is available for use in neutralization and control experiments.
Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: big potassium channel beta subunit 1; BK channel beta subunit; BK channel beta subunit 1; BK channel subunit beta-1; BKbeta; calcium-activated potassium channel beta subunit; Calcium-activated potassium channel subunit beta; Calcium-activated potassium channel subunit beta-1; Calcium-activated potassium channel, subfamily M subunit beta-1; Charybdotoxin receptor subunit beta-1; K(VCA)beta-1; large conductance Ca2+-activated K+ channel beta 1 subunit; Maxi K channel subunit beta-1; MaxiK channel beta-subunit 1; potassium channel subfamily M regulatory beta subunit 1; potassium large conductance calcium-activated channel, subfamily M, beta member 1; Slo-beta; Slo-beta-1; Slowpoke-beta
Gene Aliases: BKbeta; BKbeta1; hbeta1; hslo-beta; K(VCA)beta; k(VCA)beta-1; KCNMB1; SLO-BETA; slo-beta-1
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