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Invitrogen
Mouse IgG2a kappa Isotype Control (eBM2a), Brilliant Violet™ 480, eBioscience™
Product Details
414-4724-81
| Applications | Tested Dilution |
|---|---|
Flow Cytometry (Flow) |
Assay-dependent |
Control (Ctrl) |
Assay-dependent |
| Product Specifications | |
|---|---|
Host/Isotype |
Mouse / IgG2a, kappa |
Class |
Control |
Type |
Isotype Control |
Clone |
eBM2a |
Conjugate |
Brilliant Violet™ 480
|
Excitation/Emission Max |
437/478 nm
View spectra
|
Form |
liquid |
Concentration |
0.2 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2, with BSA |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2925642 |
Product Specific Information
Description: This is a monoclonal mouse IgG2a kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.
Applications Reported: This eBM2a antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use at the same concentration as the experimental antibody.
Brilliant Violet™ 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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