Invitrogen™

eBioscience™ 1-step Fix/Lyse Solution (10X)

Catalog number:  00-5333-54

 Related applications:

Flow Cytometry

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Description

The eBioscience 1-step Fix/Lyse Solution enables lysis of red blood cells after staining peripheral blood cells with fluorochrome-conjugated antibodies. This solution has been specially formulated to lyse non-nucleated erythrocytes while maintaining a fixed and labeled leukocyte population. Therefore, whole blood samples can be stained for the appropriate markers, RBC-lysed and fixed, washed, and then analyzed by flow cytometry. Isolation of mononuclear cells by gradient centrifugation (e.g. Ficoll-Paque density gradient separation) is not required when using this solution. The eBioscience 1-step Fix/Lyse Solution is compatible with antibodies conjugated to organic dyes and tandem dyes.

Reported Application
Flow Cytometric Analysis

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Prop-65 Warning: Control Code 65
Product Line: eBioscience™
Product Size: 50 mL
Reagent Type: Cell Lysis Buffer, Fixation Reagent

Documents

Manuals & protocols