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Myc Tag Antibody (PA5-141014) in WB
Western blot analysis of Myc Tag in different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Samples were incubated in Myc Tag Polyclonal antibody (Product # PA5-141014) using a dilution of 1:1,000 in 5% BSA at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:5,000 for 1 hour at room temperature. Positive control: Lane 1: C-terminal Myc-tagged recombinant protein; Lane 2: N-terminal Myc-tagged recombinant protein.
Product Details
PA5-141014
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human Myc aa 410-420.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932466
Target Information
Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 46-0603; avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c-myc epitope tag; Class E basic helix-loop-helix protein 39; cmyc; Myc proto-oncogene protein; Myc Tag; myc-related translation/localization regulatory factor; Proto-oncogene c-Myc; R950-25; R95025; Transcription factor p64; v-myc myelocytomatosis viral oncogene homolog
Gene Aliases: BHLHE39; c-Myc; MRTL; MYC; MYCC
UniProt ID: (Human) P01106
Entrez Gene ID: (Human) 4609
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
protein complex binding
protein dimerization activity
repressing transcription factor binding
E-box binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
branching involved in ureteric bud morphogenesis
positive regulation of mesenchymal cell proliferation
energy reserve metabolic process
chromatin remodeling
transcription from RNA polymerase II promoter
cellular iron ion homeostasis
cellular response to DNA damage stimulus
cell cycle arrest
Notch signaling pathway
positive regulation of cell proliferation
response to gamma radiation
regulation of gene expression
oxygen transport
regulation of telomere maintenance
negative regulation of stress-activated MAPK cascade
cellular response to UV
cellular response to drug
response to drug
negative regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
fibroblast apoptotic process
negative regulation of monocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
negative regulation of fibroblast proliferation
positive regulation of epithelial cell proliferation
chromosome organization
negative regulation of cell division
canonical Wnt signaling pathway
response to growth factor
positive regulation of metanephric cap mesenchymal cell proliferation
beta-catenin-TCF complex assembly
positive regulation of DNA biosynthetic process
positive regulation of response to DNA damage stimulus
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