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Invitrogen
Myc Tag Recombinant Mouse Monoclonal Antibody (A3C8-R-2)
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FIGURE: 1 / 4
Myc Tag Antibody (MA5-50639) in WB
Western blot analysis of Myc Tag in different various lysates. Lane 1:2: C-terminal Myc-tag fusion protein lysate; Lane 3/4: N-terminal Myc-tag fusion protein lysate Lysates/proteins at 50 ng/Lane. Observed band size: 34 kDa. Exposure time: 30 seconds; 12% SDS-PAGE gel. Primary antibody Myc Tag recombinant monoclonal antibody (Product # MA5-50639) with a dilution of different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody with a dilution of 1:100,000 was used for 1 hour at ro... View More
Product Details
MA5-50639
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
A3C8-R-2
Immunogen
Synthetic peptide EQKLISEEDL conjugated to keyhole limpet hemocyanin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: C-terminal Myc-tag fusion protein lysate, N-terminal Myc-tag fusion protein lysate.
Product MA5-50639 was used in an Indirect ELISA.
Target Information
Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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