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Invitrogen
NACA Polyclonal Antibody
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FIGURE: 1 / 2
NACA Antibody (40-1000) in WB
Western blot analysis was performed on whole cell extracts of SH-SY5Y (Lane 1), Caco-2 (Lane 2), A431 (Lane 3), and HEK-293 (Lane 4). The blot was probed with Anti-NACA (alpha-NAC) Rabbit Polyclonal Antibody (Product # 40-1000, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 23 kDa band corresponding to NACA (alpha-NAC) was observed across the cell lines tested. Known quantity of protein samples were electrop... View More
Product Details
40-1000
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Raised against the C-term of human NAC Alpha.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
NACA prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). It binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. NACA also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites), and may act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alpha-NAC; Alpha-NAC, muscle-specific form; Alpha-NAC/1.9.2; ANAC; NACA related; NAC-alpha; NACA; nascent polypeptide-associated complex alpha subunit; Nascent polypeptide-associated complex subunit alpha; Nascent polypeptide-associated complex subunit alpha, muscle-specific form; nascent-polypeptide-associated complex alpha polypeptide; skNAC; unnamed protein product
Gene Aliases: AL022831; AL024382; Gm1878; HSD48; mKIAA0363; NAC-alpha; NACA; NACA1; skNAC
UniProt ID: (Human) E9PAV3, (Mouse) P70670
Entrez Gene ID: (Human) 4666, (Rat) 288770, (Mouse) 17938
negative regulation of transcription from RNA polymerase II promoter
cardiac ventricle development
translation
protein targeting to membrane
negative regulation of striated muscle cell apoptotic process
protein transport
wound healing
skeletal muscle tissue regeneration
positive regulation of transcription from RNA polymerase II promoter
positive regulation of skeletal muscle tissue growth
regulation of skeletal muscle fiber development
heart trabecula morphogenesis
negative regulation of protein localization to endoplasmic reticulum
positive regulation of cell proliferation involved in heart morphogenesis
myoblast migration
negative regulation of transcription from RNA polymerase II promoter involved in heart development
positive regulation of transcription from RNA polymerase II promoter involved in heart development
regulation of nucleic acid-templated transcription
transcription, DNA-templated
regulation of transcription, DNA-templated
transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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