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Invitrogen
NAMPT Polyclonal Antibody
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FIGURE: 1 / 6
NAMPT Antibody (PA1-1045) in ICC/IF
Immunofluorescence analysis of Visfatin was done on 70% confluent log phase U87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Visfatin Rabbit Polyclonal Antibody (Product # PA1-1045) at 1 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More
Product Details
PA1-1045
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues S (472) F D E I R K N A Q L N I E L E A A H H (491) of human Visfatin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-1045 detects Visfatin (PBEF) in human samples.
PA1-1045 has been successfully used in Western blot procedures. In Western blot analysis of human adipose tissue lysate this antibody detects a ~52 kDa protein representing Visfatin.
The PA1-1045 immunogen is a synthetic peptide corresponding to residues S (472) F D E I R K N A Q L N I E L E A A H H (491) of human Visfatin.
Target Information
Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp666B131; EC 2.4.2.12; MGC117256; NAmPRTase; Nicotinamide phosphoribosyltransferase; Pre-B cell-enhancing factor; Pre-B-cell colony-enhancing factor 1; unnamed protein product; Visfatin
Gene Aliases: 1110035O14Rik; NAMPT; PBEF; PBEF1; VF; VISFATIN
UniProt ID: (Human) P43490
Entrez Gene ID: (Human) 10135
NADP biosynthetic process
nicotinamide metabolic process
inflammatory response
signal transduction
cell-cell signaling
circadian rhythm
positive regulation of cell proliferation
insulin receptor signaling pathway
NAD biosynthetic process
positive regulation of gene expression
pyridine nucleotide biosynthetic process
circadian regulation of gene expression
NAD salvage
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
adipose tissue development
positive regulation of ERK1 and ERK2 cascade
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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