Search
Invitrogen
NAMPT Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 8
NAMPT Antibody (PA5-85535) in ICC/IF
NAMPT Polyclonal Antibody detects Visfatin protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Visfatin protein stained by NAMPT Polyclonal Antibody (Product # PA5-85535) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-85535
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Visfatin. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (92%), Rat (89%), Pig (94%), Chicken (90%), Bovine (96%).
Positive Control: 293T, A431, K562, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DKFZp666B131; EC 2.4.2.12; MGC117256; NAmPRTase; Nicotinamide phosphoribosyltransferase; PBEF; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; Pre-B-cell colony-enhancing factor 1 homolog; unnamed protein product; Visfatin
Gene Aliases: 1110035O14Rik; AI314458; AI480535; NAmPRTase; NAMPT; PBEF; PBEF1; VF; VISFATIN
UniProt ID: (Human) P43490, (Mouse) Q99KQ4, (Rat) Q80Z29
Entrez Gene ID: (Human) 10135, (Mouse) 59027, (Rat) 297508
cytokine activity
protein binding
transferase activity
transferase activity, transferring glycosyl groups
identical protein binding
nicotinamide phosphoribosyltransferase activity
nicotinate-nucleotide diphosphorylase (carboxylating) activity
drug binding
protein homodimerization activity
transferase
NADP biosynthetic process
nicotinamide metabolic process
inflammatory response
signal transduction
cell-cell signaling
circadian rhythm
positive regulation of cell proliferation
insulin receptor signaling pathway
NAD biosynthetic process
positive regulation of gene expression
pyridine nucleotide biosynthetic process
circadian regulation of gene expression
NAD salvage
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
adipose tissue development
positive regulation of ERK1 and ERK2 cascade
positive regulation of smooth muscle cell proliferation
positive regulation of nitric-oxide synthase biosynthetic process
female pregnancy
response to organic cyclic compound
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support