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Proteintech
NANOG Polyclonal Antibody, CoraLite® Plus 488
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FIGURE: 1 / 1
NANOG Antibody (CL488-14295) in Flow
1x10^6 NCCIT cells were intracellularly stained with 0.4 µg CoraLite® Plus 488 Anti-Human NANOG (CL488-14295) (red), or 0.4 µg CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Product Details
CL488-14295
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
NANOG fusion protein Ag5645
Conjugate
Excitation/Emission Max
493/522 nm
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.5% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, store in dark
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: MSVDPACPQ SLPCFEASDC KESSPMPVIC GPEENYPSLQ MSSAEMPHTE TVSPLPSSMD LLIQDSPDSS TSPKGKQPTS AEKSVAKKED KVPVKKQKTR TVFSSTQLCV LNDRFQRQKY LSLQQMQELS NILNLSYKQV KTWFQNQRMK SKRWQKNNWP KNSNGVTQKA SAPTYPSLYS SYHQGCLVNP TGNLPMWSNQ TWNNSTWSNQ TQNIQSWSNH SWNTQTWCTQ SWNNQAWNSP FYNCGEESLQ SCMQFQPNSP ASDLEAALEA AGEGLNVIQQ TTRYFSTPQT MDLFLNYSMN MQPEDV
Target Information
NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: early embryo specific expression NK family; Early embryo specific expression NK-type homeobox protein; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
Gene Aliases: 2410002E02Rik; Ecat4; ENK; NANOG
UniProt ID: (Human) Q9H9S0, (Mouse) Q80Z64
Entrez Gene ID: (Human) 79923, (Mouse) 71950, (Rat) 414065
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
sequence-specific DNA binding
enhancer sequence-specific DNA binding
chromatin binding
transcription regulatory region DNA binding
molecular_function
homeodomain transcription factor
endodermal cell fate specification
transcription, DNA-templated
regulation of transcription, DNA-templated
cell proliferation
regulation of gene expression
stem cell population maintenance
cell differentiation
somatic stem cell population maintenance
regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of nucleic acid-templated transcription
negative regulation of transcription from RNA polymerase II promoter
mesodermal cell fate commitment
multicellular organism development
positive regulation of cell proliferation
embryo development
response to organic substance
negative regulation of cell fate commitment
stem cell division
negative regulation of BMP signaling pathway
response to retinoic acid
negative regulation of endodermal cell fate specification
negative regulation of cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of mitotic cell cycle
stem cell differentiation
gonad development
multidimensional cell growth
embryonic pattern specification
response to organic cyclic compound
cellular response to erythropoietin
tissue regeneration
cellular response to rapamycin
replicative senescence
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