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NAP1L1 Polyclonal Antibody

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Antibody Testing Data (30)

NAP1L1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 30

NAP1L1 Antibody (14898-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using NAP1L1 antibody 14898-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Product Image Gallery

NAP1L1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

NAP1L1 Antibody in Western Blot (WB) — Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

NAP1L1 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug NAP1L1 antibody (14898-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

NAP1L1 Antibody in Immunoprecipitation (IP) — IP result of anti-NAP1L1 (IP:14898-1-AP, 4ug; Detection:14898-1-AP 1:1000) with HEK-293 cells lysate 800ug.

Product Details

14898-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NAP1L1 Fusion Protein Ag6690 (1-391 aa encoded by BC002387)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MADIDNKEQ SELDQDLDDV EEVEEEETGE ETKLKARQLT VQMMQNPQIL AALQERLDGL VETPTGYIES LPRVVKRRVN ALKNLQVKCA QIEAKFYEEV HDLERKYAVL YQPLFDKRFE IINAIYEPTE EECEWKPDEE DEISEELKEK AKIEDEKKDE EKEDPKGIPE FWLTVFKNVD LLSDMVQEHD EPILKHLKDI KVKFSDAGQP MSFVLEFHFE PNEYFTNEVL TKTYRMRSEP DDSDPFSFDG PEIMGCTGCQ IDWKKGKNVT LKTIKKKQKH KGRGTVRTVT KTVSNDSFFN FFAPPEVPES GDLDDDAEAI LAADFEIGHF LRERIIPRSV LYFTGEAIED DDDDYDEEGE EADEEGEEEG DEENDPDYDP KKDQNPAECK QQ (1-391 aa encoded by BC002387)

Target Information

NAP1L1 is a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NAP1L1 is a novel microtubule-associated protein.

Cytoskeleton (Hoboken, N.J.)

Peng N,Zhang Y,Zhang X,Wu HY,Nakamura F

Wed Apr 26 00:00:00 EDT 2023

NAP1L1 is a novel microtubule-associated protein.

Cytoskeleton (Hoboken, N.J.)

Peng N,Zhang Y,Zhang X,Wu HY,Nakamura F

Wed Apr 26 00:00:00 EDT 2023

CRISPR-assisted transcription activation by phase separation proteins.

Protein & cell

Liu J,Chen Y,Nong B,Luo X,Cui K,Tan W,Yang Y,Ma W,Liang P,Songyang Z

Sat Mar 11 00:00:00 EST 2023

Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage.

Human reproduction (Oxford, England)

Wang X,Shi W,Zhao S,Gong D,Li S,Hu C,Chen ZJ,Li Y,Yan J

Tue May 02 00:00:00 EDT 2023

Mechanism of client selection by the protein quality-control factor UBE2O.

Nature structural & molecular biology

Yip MCJ,Sedor SF,Shao S

Mon Aug 01 00:00:00 EDT 2022

NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.

Cancer cell international

Liu S,Zhang Y,Cui S,Song D,Li B,Chen Q,Yao G,Gong B

Sat Nov 13 00:00:00 EST 2021

NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.

Cancer cell international

Liu S,Zhang Y,Cui S,Song D,Li B,Chen Q,Yao G,Gong B

Sat Nov 13 00:00:00 EST 2021

NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.

Cancer cell international

Liu S,Zhang Y,Cui S,Song D,Li B,Chen Q,Yao G,Gong B

Sat Nov 13 00:00:00 EST 2021

Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.

Journal of dermatological science

Mizuhashi S,Fukushima S,Ishibashi T,Kuriyama H,Kimura T,Kanemaru H,Kajihara I,Makino K,Miyashita A,Aoi J,Kita K,Ihn H

Thu Apr 01 00:00:00 EDT 2021

NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.

Frontiers in cell and developmental biology

Zhang YW,Chen Q,Li B,Li HY,Zhao XK,Xiao YY,Liu S,Zuo S

Tue Aug 10 00:00:00 EDT 2021

NAP1L1 and NAP1L4 Binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein Is Bivalent and Requires Phosphorylation.

Journal of virology

Dominguez F,Shiliaev N,Lukash T,Agback P,Palchevska O,Gould JR,Meshram CD,Prevelige PE,Green TJ,Agback T,Frolova EI,Frolov I

Mon Jul 26 00:00:00 EDT 2021

NAP1L1 and NAP1L4 Binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein Is Bivalent and Requires Phosphorylation.

Journal of virology

Dominguez F,Shiliaev N,Lukash T,Agback P,Palchevska O,Gould JR,Meshram CD,Prevelige PE,Green TJ,Agback T,Frolova EI,Frolov I

Mon Jul 26 00:00:00 EDT 2021

Nucleosome Assembly Protein 1-Like 1 (Nap1l1) Regulates the Proliferation of Murine Induced Pluripotent Stem Cells.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Yan Y,Yin P,Gong H,Xue Y,Zhang G,Fang B,Chen Z,Li Y,Yang C,Huang Z,Yang X,Ge J,Zou Y

Tue Oct 25 00:00:00 EDT 2016

Nucleosome Assembly Protein 1-Like 1 (Nap1l1) Regulates the Proliferation of Murine Induced Pluripotent Stem Cells.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Yan Y,Yin P,Gong H,Xue Y,Zhang G,Fang B,Chen Z,Li Y,Yang C,Huang Z,Yang X,Ge J,Zou Y

Tue Oct 25 00:00:00 EDT 2016

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Oncotarget

Zhang L,Yu Z,Wang Y,Wang X,Zhang L,Wang C,Yue Q,Wang X,Deng S,Huo X,Tian X,Huang S,Zhang B,Ma X

Tue Nov 22 00:00:00 EST 2016

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Oncotarget

Zhang L,Yu Z,Wang Y,Wang X,Zhang L,Wang C,Yue Q,Wang X,Deng S,Huo X,Tian X,Huang S,Zhang B,Ma X

Tue Nov 22 00:00:00 EST 2016

Bioinformatics

Protein Aliases: Brain protein DN38; hNRP; HSP22-like protein interacting protein; NAP-1-related protein; Nucleosome assembly protein 1-like 1; nucleosome assembly protein-1

Gene Aliases: AA407126; AI256722; D10Ertd68e; NAP-1; NAP1; NAP1L; NAP1L1; NRP

UniProt ID: (Human) P55209, (Rat) Q9Z2G8, (Mouse) P28656

Entrez Gene ID: (Human) 4673, (Rat) 89825, (Mouse) 53605

Molecular Function: chromatin/chromatin-binding, or -regulatory protein