|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 107 and 408 of Human NAPRT1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-31880 targets NAPRT1 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-31880 immunogen is recombinant protein fragment corresponding to a region within amino acids 107 and 408 of Human NAPRT1.
Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 188.8.131.52) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
NAMPT is the cellular target of STF-31-like small-molecule probes.
PA5-31880 was used in western blot to identify the importance of the NAMPT as the target for small molecules like STF-31
|Adams DJ,Ito D,Rees MG,Seashore-Ludlow B,Puyang X,Ramos AH,Cheah JH,Clemons PA,Warmuth M,Zhu P,Shamji AF,Schreiber SL||ACS chemical biology (9:2247)||2014|