Proteintech

NAT10 Polyclonal Antibody

View all (9) NAT10 antibodies

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Questions & Answers

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NAT10 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Hela cells, using NAT10 antibody 13365-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

NAT10 Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13365-1-AP (NAT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

NAT10 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13365-1-AP (NAT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

NAT10 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 13365-1-AP (NAT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

NAT10 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 13365-1-AP ( NAT10 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

NAT10 Antibody in Immunoprecipitation (IP) — IP result of anti-NAT10 (IP:13365-1-AP, 2ug; Detection:13365-1-AP 1:500) with mouse testis tissue lysate 4000ug.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:20-1:200

-

Immunofluorescence (IF)

1:20-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Western Blot (WB)

1:1000-1:6000

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NAT10 Fusion Protein Ag4184

Conjugate

Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

The specificity of this antibody has been tested by siRNA.

Target Information

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 18S rRNA cytosine acetyltransferase; hALP; N-acetyltransferase 10; N-acetyltransferase 10 (GCN5-related); N-acetyltransferase-like protein; novel putative ATPase domain containing protein; RNA cytidine acetyltransferase

Gene Aliases: AI429152; ALP; KIAA1709; NAT10; NET43; RGD1306717

UniProt ID: (Human) Q9H0A0, (Mouse) Q8K224

Entrez Gene ID: (Human) 55226, (Mouse) 98956, (Rat) 311257

rRNA modification biological_process negative regulation of telomere maintenance via telomerase ribosomal small subunit biogenesis tRNA acetylation metabolic process

Process(es)

protein binding ATP binding N-acetyltransferase activity poly(A) RNA binding nucleotide binding dihydrolipoamide branched chain acyltransferase activity sterol O-acyltransferase activity O-acyltransferase activity palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity carnitine O-acyltransferase activity acetyltransferase activity C-acyltransferase activity palmitoyltransferase activity N-acyltransferase activity acylglycerol O-acyltransferase activity serine O-acyltransferase activity O-acetyltransferase activity O-octanoyltransferase activity octanoyltransferase activity O-palmitoyltransferase activity S-acyltransferase activity S-acetyltransferase activity S-malonyltransferase activity malonyltransferase activity C-palmitoyltransferase activity transferase activity transferase activity, transferring acyl groups succinyltransferase activity N-succinyltransferase activity O-succinyltransferase activity S-succinyltransferase activity sinapoyltransferase activity O-sinapoyltransferase activity peptidyl-lysine N6-myristoyltransferase activity peptidyl-lysine N6-palmitoyltransferase activity benzoyl acetate-CoA thiolase activity 3-hydroxybutyryl-CoA thiolase activity 3-ketopimelyl-CoA thiolase activity N-palmitoyltransferase activity myristoyltransferase activity acyl-CoA N-acyltransferase activity protein-cysteine S-myristoyltransferase activity protein-cysteine S-acyltransferase activity dihydrolipoamide S-acyltransferase activity glucosaminyl-phosphotidylinositol O-acyltransferase activity ergosterol O-acyltransferase activity lanosterol O-acyltransferase activity naphthyl-2-oxomethyl-succinyl-CoA succinyl transferase activity 2,4,4-trimethyl-3-oxopentanoyl-CoA 2-C-propanoyl transferase activity 2-methylhexanoyl-CoA C-acetyltransferase activity butyryl-CoA 2-C-propionyltransferase activity 2,6-dimethyl-5-methylene-3-oxo-heptanoyl-CoA C-acetyltransferase activity L-2-aminoadipate N-acetyltransferase activity UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase activity keto acid formate lyase activity Ras palmitoyltransferase activity azetidine-2-carboxylic acid acetyltransferase activity peptidyl-lysine N-acetyltransferase activity, acting on acetyl phosphate as donor acetyl-CoA:L-lysine N6-acetyltransferase