Invitrogen

NAT13 Polyclonal Antibody

View all (9) NAT13 antibodies

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NAT13 Antibody in Immunocytochemistry (ICC) — Immunofluorescence analysis of A549 cells using NAA50 Polyclonal antibody (Product # PA5-92334). Blue: DAPI for nuclear staining.

NAT13 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human stomach using NAA50 Polyclonal antibody (Product # PA5-92334) at a dilution of 1:200 (40x lens).

NAT13 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse heart using NAA50 Polyclonal antibody (Product # PA5-92334) at a dilution of 1:200 (40x lens).

NAT13 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded rat kidney using NAA50 Polyclonal antibody (Product # PA5-92334) at a dilution of 1:200 (40x lens).

NAT13 Antibody in Western Blot (WB) — Western blot analysis of extracts of various cell lines, using NAA50 Polyclonal antibody (Product # PA5-92334) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Product Details

Tested Applications

Dilution

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

1:200-1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human NAA50

Conjugate

Unconjugated

Form

liquid

Concentration

2.55 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: MKGSRIELGD VTPHNIKQLK RLNQVIFPVS YNDKFYKDVL EVGELAKLAY FNDIAVGAVC CRVDHSQNQK RLYIMTLGCL APYRRLGIGT KMLNHVLNIC EKDGTFDNIY LHVQISNESA IDFYRKFGFE IIETKKNYYK RIEPADAHVL QKNLKVPSGQ NADVQKTDN; Positive Samples: SKOV3, HL-60, 293T, MCF7; Cellular Location: Cytoplasm

Target Information

NAT13 is probable catalytic component of the ARD1A-NARG1 complex which displays alpha (N-terminal) acetyltransferase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: hNaa50p; hNAT5; hSAN; Mak3 homolog; Mak3p homolog; N-acetyltransferase 13; N-acetyltransferase 13 (GCN5-related); N-acetyltransferase 5; N-acetyltransferase NAT13; N-acetyltransferase san homolog; N-alpha-acetyltransferase 50; N-alpha-acetyltransferase 50, NatE catalytic subunit; N-epsilon-acetyltransferase 50; Nalpha acetyltransferase 50; NatE catalytic subunit

Gene Aliases: 2600005K24Rik; 2810441M03Rik; AW112078; MAK3; Mak3p; NAA50; NAT13; NAT13P; NAT5; NAT5P; SAN

UniProt ID: (Human) Q9GZZ1, (Mouse) Q6PGB6

Entrez Gene ID: (Human) 80218, (Rat) 288108, (Mouse) 72117

Molecular Function: acetyltransferase transferase

N-terminal protein amino acid acetylation histone H4 acetylation mitotic sister chromatid cohesion, centromeric

Process(es)

peptide alpha-N-acetyltransferase activity protein binding H4 histone acetyltransferase activity peptidyl-lysine N-acetyltransferase activity, acting on acetyl phosphate as donor dihydrolipoamide branched chain acyltransferase activity sterol O-acyltransferase activity N-acetyltransferase activity O-acyltransferase activity palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity carnitine O-acyltransferase activity acetyltransferase activity C-acyltransferase activity palmitoyltransferase activity N-acyltransferase activity acylglycerol O-acyltransferase activity serine O-acyltransferase activity O-acetyltransferase activity O-octanoyltransferase activity octanoyltransferase activity O-palmitoyltransferase activity S-acyltransferase activity S-acetyltransferase activity S-malonyltransferase activity malonyltransferase activity C-palmitoyltransferase activity transferase activity transferase activity, transferring acyl groups succinyltransferase activity N-succinyltransferase activity O-succinyltransferase activity S-succinyltransferase activity sinapoyltransferase activity O-sinapoyltransferase activity peptidyl-lysine N6-myristoyltransferase activity peptidyl-lysine N6-palmitoyltransferase activity benzoyl acetate-CoA thiolase activity 3-hydroxybutyryl-CoA thiolase activity 3-ketopimelyl-CoA thiolase activity N-palmitoyltransferase activity myristoyltransferase activity acyl-CoA N-acyltransferase activity protein-cysteine S-myristoyltransferase activity protein-cysteine S-acyltransferase activity dihydrolipoamide S-acyltransferase activity glucosaminyl-phosphotidylinositol O-acyltransferase activity ergosterol O-acyltransferase activity lanosterol O-acyltransferase activity naphthyl-2-oxomethyl-succinyl-CoA succinyl transferase activity 2,4,4-trimethyl-3-oxopentanoyl-CoA 2-C-propanoyl transferase activity 2-methylhexanoyl-CoA C-acetyltransferase activity butyryl-CoA 2-C-propionyltransferase activity 2,6-dimethyl-5-methylene-3-oxo-heptanoyl-CoA C-acetyltransferase activity L-2-aminoadipate N-acetyltransferase activity UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase activity keto acid formate lyase activity Ras palmitoyltransferase activity azetidine-2-carboxylic acid acetyltransferase activity acetyl-CoA:L-lysine N6-acetyltransferase