|Tested species reactivity||Hamster, Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Partial length human NBS1 protein [Swiss-Prot #O60934]|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-Dependent|
|Flow Cytometry (Flow)||Assay-Dependent|
|Immunoprecipitation (IP)||3 ul|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ICC/IF has been performed using methanol-fixed IMR90 primary human fibroblasts.
Suggested positive control: antigen standard for NBN (transient overexpression lysate), TK6 and HeLa whole cell extract.
The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.