Proteintech

NCBP1 Polyclonal Antibody

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NCBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 10349-1-AP (NCBP1 antibody) at dilution of 1:100 (under 10x lens).

NCBP1 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

NCBP1 Antibody in Immunoprecipitation (IP) — IP result of anti-NCBP1 (IP:10349-1-AP, 4ug; Detection:10349-1-AP 1:500) with HeLa cells lysate 2480ug.

Product Details

10349-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 6 publications 6 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Artificial Control, Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NCBP1 Fusion Protein Ag0496 (46-350 aa encoded by BC001450) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.17 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: LESNLEGLA GVLEADLPNY KSKILRLLCT VARLLPEKLT IYTTLVGLLN ARNYNFGGEF VEAMIRQLKE SLKANNYNEA VYLVRFLSDL VNCHVIAAPS MVAMFENFVS VTQEEDVPQV RRDWYVYAFL SSLPWVGKEL YEKKDAEMDR IFANTESYLK RRQKTHVPML QVWTADKPHP QEEYLDCLWA QIQKLKKDRW QERHILRPYL AFDSILCEAL QHNLPPFTPP PHTEDSVYPM PRVIFRMFDY TDDPEGPVMP GSHSVERFVI EENLHCIIKS HWKERKTCAA QLVSYPGKNK IPLNYH (46-350 aa encoded by BC001450)

Target Information

May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.

Nucleic acids research

Du Y,Hou G,Zhang H,Dou J,He J,Guo Y,Li L,Chen R,Wang Y,Deng R,Huang J,Jiang B,Xu M,Cheng J,Chen GQ,Zhao X,Yu J

Fri Jun 01 00:00:00 EDT 2018

RNA-methylation-dependent RNA processing controls the speed of the circadian clock.

Cell

Fustin JM,Doi M,Yamaguchi Y,Hida H,Nishimura S,Yoshida M,Isagawa T,Morioka MS,Kakeya H,Manabe I,Okamura H

Thu Nov 07 00:00:00 EST 2013

Heat shock protein 90 regulates phosphatidylinositol 3-kinase-related protein kinase family proteins together with the RUVBL1/2 and Tel2-containing co-factor complex.

Cancer science

Izumi N,Yamashita A,Hirano H,Ohno S

Sun Jan 01 00:00:00 EST 2012

AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.

Science signaling

Izumi N,Yamashita A,Iwamatsu A,Kurata R,Nakamura H,Saari B,Hirano H,Anderson P,Ohno S

Tue Apr 06 00:00:00 EDT 2010

SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.

Genes & development

Yamashita A,Izumi N,Kashima I,Ohnishi T,Saari B,Katsuhata Y,Muramatsu R,Morita T,Iwamatsu A,Hachiya T,Kurata R,Hirano H,Anderson P,Ohno S

Fri May 01 00:00:00 EDT 2009

SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.

Genes & development

Yamashita A,Izumi N,Kashima I,Ohnishi T,Saari B,Katsuhata Y,Muramatsu R,Morita T,Iwamatsu A,Hachiya T,Kurata R,Hirano H,Anderson P,Ohno S

Fri May 01 00:00:00 EDT 2009

Chapter 9. Studying nonsense-mediated mRNA decay in mammalian cells.

Methods in enzymology

Matsuda D,Sato H,Maquat LE

Thu Mar 26 00:00:00 EDT 2009

Chapter 9. Studying nonsense-mediated mRNA decay in mammalian cells.

Methods in enzymology

Matsuda D,Sato H,Maquat LE

Thu Mar 26 00:00:00 EDT 2009

Chapter 9. Studying nonsense-mediated mRNA decay in mammalian cells.

Methods in enzymology

Matsuda D,Sato H,Maquat LE

Thu Mar 26 00:00:00 EDT 2009

Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay.

Molecular cell

Sato H,Hosoda N,Maquat LE

Fri Feb 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: 80 kDa nuclear cap-binding protein; CBP80; MGC2087; NCBP 80 kDa subunit; nuclear cap binding protein subunit 1, 80kD; nuclear cap binding protein subunit 1, 80kDa; Nuclear cap-binding protein subunit 1; RP11-546O6.1

Gene Aliases: AU014645; AW538051; CBP80; NCBP; NCBP1; Sto1

UniProt ID: (Human) Q09161, (Mouse) Q3UYV9

Entrez Gene ID: (Human) 4686, (Mouse) 433702

Molecular Function: RNA metabolism protein RNA processing factor RNA splicing factor nucleic acid metabolism protein