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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 185 of Human NCE2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31221 targets NCE2 in WB applications and shows reactivity with Human samples.
The PA5-31221 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 185 of Human NCE2.
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
NCE2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; ubiquitin conjugating enzyme E2F (putative); ubiquitin-conjugating enzyme E2 F; ubiquitin-conjugating enzyme E2F (putative)