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Invitrogen
NCF2 Polyclonal Antibody
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FIGURE: 1 / 2
NCF2 Antibody (PA5-88253) in IHC (P)
Immunohistochemistry analysis of NCF2 in paraffin-embedded human esophageal cancer. Samples were incubated with NCF2 Polyclonal antibody (Product # PA5-88253) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-88253
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human NOXA2/p67phox (NP_0004242)
Conjugate
Form
Liquid
Concentration
7.65 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PPPRPKTPEI FRALEGEAHR VLFGFVPETK EELQVMPGNI VFVLKKGNDN WATVMFNGQK GLVPCNYLEP VELRIHPQQQ PQEESSPQSD IPAPPSSKAP GRPQLSPGQK QKEEPKEVKL SVPMPYTLKV HYKYTVVMKT QPGLPYSQVR DMVSKKLELR LEHTKLSYRP RDSNELVPLS EDSMKDAWGQ VKNYCLTLWC ENTVGDQGFP DEPKESEKAD ANNQTTEPQL KKGSQVEALF SYEATQPEDL EFQEGDIILV LSKVNEEWLE GECKGKVGIF PKVFVEDCAT TDLESTRREV; Positive Samples: THP-1, Mouse spleen; Cellular Location: Cytoplasm
Target Information
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 67 kDa neutrophil oxidase factor; chronic granulomatous disease, autosomal 2; FLJ93058; NADPH oxidase activator 2; NADPH oxidase subunit (67 kD); NADPH oxidase subunit (67kDa); NCF-2; ncf2; Neutrophil cytosol factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2); Neutrophil NADPH oxidase factor 2; p67-phox
Gene Aliases: NCF-2; NCF2; NOXA2; P67-PHOX; P67PHOX
UniProt ID: (Human) P19878, (Mouse) O70145
Entrez Gene ID: (Human) 4688, (Mouse) 17970
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
superoxide metabolic process
cellular defense response
superoxide anion generation
innate immune response
cell redox homeostasis
respiratory burst
vascular endothelial growth factor receptor signaling pathway
oxidation-reduction process
NADP catabolic process
response to glucose
response to organic cyclic compound
response to lipopolysaccharide
cellular response to hormone stimulus
positive regulation of neuron apoptotic process
positive regulation of blood pressure
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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