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Proteintech

NCK1 Polyclonal Antibody

3 References
View all (11) NCK1 antibodies
Datasheet
Protocols
Questions & Answers
Tech Support
Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

15247-1-AP

Applications Tested Dilution Publications

Western Blot (WB)

 1:500-1:2,000 View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

 1:50-1:500 -

Immunocytochemistry (ICC/IF)

 1:10-1:100 -

Flow Cytometry (Flow)

 0.2 µg/1x10^6 cells -

Immunoprecipitation (IP)

 0.5-4.0 µg View 1 publication 1 publication
Product Specifications

Species Reactivity

 Human, Mouse, Rat

Published species

 Human

Host/Isotype

 Rabbit / IgG

Class

 Polyclonal

Type

 Antibody

Immunogen

NCK1 Fusion Protein Ag0979 (84-377 aa encoded by BC006403)
View immunogen

Conjugate

 Unconjugated

Form

 Liquid

Concentration

 0.21 mg/mL

Purification

 Antigen affinity chromatography

Storage buffer

 PBS, pH 7.3, with 50% glycerol

Contains

 0.02% sodium azide

Storage conditions

 -20°C

Product Specific Information

Immunogen sequence: PSVPDSASP ADDSFVDPGE RLYDLNMPAY VKFNYMAERE DELSLIKGTK VIVMEKCSDG WWRGSYNGQV GWFPSNYVTE EGDSPLGDHV GSLSEKLAAV VNNLNTGQVL HVVQALYPFS SSNDEELNFE KGDVMDVIEK PENDPEWWKC RKINGMVGLV PKNYVTVMQN NPLTSGLEPS PPQCDYIRPS LTGKFAGNPW YYGKVTRHQA EMALNERGHE GDFLIRDSES SPNDFSVSLK AQGKNKHFKV QLKETVYCIG QRKFSTMEEL VEHYKKAPIF TSEQGEKLYL VKHLS (84-377 aa encoded by B C006403)

Target Information

NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cytoplasmic protein NCK1; melanoma NCK protein; MGC12668; NCK adaptor protein 1; NCK tyrosine kinase; Nck-1; non-catalytic region of tyrosine kinase; non-catalytic region of tyrosine kinase adaptor protein 1; SH2/SH3 adaptor protein NCK-alpha

Gene Aliases: 6330586M15Rik; D230010O13Rik; NCK; Nck-1; NCK1; NCKalpha

UniProt ID: (Human) P16333, (Mouse) Q99M51

Entrez Gene ID: (Human) 4690, (Rat) 300955, (Mouse) 17973

Molecular Function: scaffold/adaptor protein

Function(s)
negative regulation of protein kinase activity substrate-dependent cell migration, cell extension actin filament organization signal complex assembly vesicle-mediated transport lamellipodium assembly positive regulation of actin filament polymerization negative regulation of peptidyl-serine phosphorylation positive regulation of translation in response to endoplasmic reticulum stress Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of T cell proliferation T cell activation positive regulation of GTPase activity positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway T cell receptor signaling pathway response to other organism negative regulation of cell death peptidyl-serine dephosphorylation positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of cap-dependent translational initiation positive regulation of cap-independent translational initiation negative regulation of PERK-mediated unfolded protein response negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress cell migration positive regulation of translation in response to stress regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation negative regulation of endoplasmic reticulum unfolded protein response regulation of translation
Process(es)
protein kinase inhibitor activity SH3/SH2 adaptor activity ARF guanyl-nucleotide exchange factor activity receptor binding protein binding cytoskeletal adaptor activity protein domain specific binding receptor signaling complex scaffold activity protein binding, bridging receptor tyrosine kinase binding eukaryotic initiation factor eIF2 binding cadherin binding involved in cell-cell adhesion protein kinase regulator activity
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