Proteintech

NCK1 Polyclonal Antibody

View all (11) NCK1 antibodies

Questions & Answers

Questions & Answers

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NCK1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using NCK1 antibody 15247-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

NCK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15247-1-AP (NCK1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NCK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15247-1-AP (NCK1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NCK1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NCK1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NCK1 Antibody in Western Blot (WB) — Rat brain tissue were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NCK1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

NCK1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug NCK1 antibody (15247-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

NCK1 Antibody in Immunoprecipitation (IP) — IP result of anti-NCK1 (IP:15247-1-AP, 4ug; Detection:15247-1-AP 1:500) with mouse brain tissue lysate 2640ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

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Immunocytochemistry (ICC/IF)

1:10-1:100

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Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

-

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NCK1 Fusion Protein Ag0979

Conjugate

Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cytoplasmic protein NCK1; melanoma NCK protein; MGC12668; NCK adaptor protein 1; NCK tyrosine kinase; Nck-1; non-catalytic region of tyrosine kinase; non-catalytic region of tyrosine kinase adaptor protein 1; SH2/SH3 adaptor protein NCK-alpha

Gene Aliases: 6330586M15Rik; D230010O13Rik; NCK; Nck-1; NCK1; NCKalpha

UniProt ID: (Human) P16333, (Mouse) Q99M51

Entrez Gene ID: (Human) 4690, (Rat) 300955, (Mouse) 17973

Molecular Function: scaffold/adaptor protein

negative regulation of protein kinase activity substrate-dependent cell migration, cell extension actin filament organization signal complex assembly vesicle-mediated transport lamellipodium assembly positive regulation of actin filament polymerization negative regulation of peptidyl-serine phosphorylation positive regulation of translation in response to endoplasmic reticulum stress Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of T cell proliferation T cell activation positive regulation of GTPase activity positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway T cell receptor signaling pathway response to other organism negative regulation of cell death peptidyl-serine dephosphorylation positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of cap-dependent translational initiation positive regulation of cap-independent translational initiation negative regulation of PERK-mediated unfolded protein response negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress cell migration positive regulation of translation in response to stress regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation negative regulation of endoplasmic reticulum unfolded protein response regulation of translation

Process(es)

protein kinase inhibitor activity SH3/SH2 adaptor activity ARF guanyl-nucleotide exchange factor activity receptor binding protein binding cytoskeletal adaptor activity protein domain specific binding receptor signaling complex scaffold activity protein binding, bridging receptor tyrosine kinase binding eukaryotic initiation factor eIF2 binding cadherin binding involved in cell-cell adhesion protein kinase regulator activity