Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 2 publications 2 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:500 | - |
Immunocytochemistry (ICC/IF) |
1:10-1:100 | - |
Flow Cytometry (Flow) |
0.2 µg/1x10^6 cells | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
NCK1 Fusion Protein Ag0979 (84-377 aa encoded by BC006403) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.21 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: PSVPDSASP ADDSFVDPGE RLYDLNMPAY VKFNYMAERE DELSLIKGTK VIVMEKCSDG WWRGSYNGQV GWFPSNYVTE EGDSPLGDHV GSLSEKLAAV VNNLNTGQVL HVVQALYPFS SSNDEELNFE KGDVMDVIEK PENDPEWWKC RKINGMVGLV PKNYVTVMQN NPLTSGLEPS PPQCDYIRPS LTGKFAGNPW YYGKVTRHQA EMALNERGHE GDFLIRDSES SPNDFSVSLK AQGKNKHFKV QLKETVYCIG QRKFSTMEEL VEHYKKAPIF TSEQGEKLYL VKHLS (84-377 aa encoded by B C006403)
NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cytoplasmic protein NCK1; melanoma NCK protein; MGC12668; NCK adaptor protein 1; NCK tyrosine kinase; Nck-1; non-catalytic region of tyrosine kinase; non-catalytic region of tyrosine kinase adaptor protein 1; SH2/SH3 adaptor protein NCK-alpha
Gene Aliases: 6330586M15Rik; D230010O13Rik; NCK; Nck-1; NCK1; NCKalpha
UniProt ID: (Human) P16333, (Mouse) Q99M51
Entrez Gene ID: (Human) 4690, (Rat) 300955, (Mouse) 17973
Molecular Function:
scaffold/adaptor protein
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