Invitrogen

NCKAP1 Polyclonal Antibody

View all (10) NCKAP1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)

FIGURE: 1 / 1

NCKAP1 Antibody (PA5-88433) in WB

Western blot analysis of NCKAP1 in extracts of various cell lines. Samples were incubated with NCKAP1 Polyclonal antibody (Product # PA5-88433) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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Product Details

PA5-88433

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NCKAP1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.52 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2804908

Product Specific Information

Immunogen sequence: MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKACGD PKAKPSYLID KNLESAVKFI VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV MEFKDHVCEL LNTIDVCQVF FDITVNFDLT KNYLDLIITY TTLMILLSRI EERKAIIGLY NYAHEMTHGA SDREYPRLGQ MIVDYENPLK KMMEEFVPHS KSLSDALISL QMVYPRRNLS ADQWRNAQLL SLISAPSTML NPAQSDTMPC; Positive Samples: U-87MG, HeLa, MCF7, HepG2, Mouse heart, Rat brain; Cellular Location: Cell membrane, Cell projection, Cytoplasmic side, Single-pass membrane protein, lamellipodium membrane

Target Information

This gene interacts with Nck and may be involved in the downstream signaling of Nck and shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking. Complementation studies show that Dhm1 has a similar function in mouse as dhp1. The function of the human gene has not yet been determined. Transcript variants encoding different isoforms have been noted for this gene; however, their full-length nature is not known.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.