PA1-844A detects human and mouse nuclear receptor corepressor (N-CoR).
PA1-844A has been successfully used in Western blot, ICC/IF, IHC (P) and immunoprecipitation (ChIP) procedures. By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages. This antibody is not recommended for mouse C2C12 cells in immunofluorescent applications.
The PA1-844A immunogen is a synthetic peptide corresponding to residues C P(2427) A P L L S A Q Y E T L S D S D D(2443) of mouse N-CoR. This immunizing peptide (Cat. # PEP-061) is available for use in neutralization and control experiments.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hCIT529I10; KIAA1047; N-CoR; N-CoR1; NCOR1; Nuclear Receptor Corepressor; Nuclear receptor corepressor 1; protein phosphatase 1, regulatory subunit 109; retinoid X receptor interacting protein 13; retinoid X receptor-interacting protein 13; thyroid hormone- and retinoic acid receptor-associated corepressor 1; TRAC1
Gene Aliases: 5730405M06Rik; A230020K14Rik; hN-CoR; KIAA1047; mKIAA1047; N-CoR; N-CoR1; NCOR1; PPP1R109; RIP13; Rxrip13; TRAC1
UniProt ID: (Human) O75376