Invitrogen

NCoR2 Polyclonal Antibody

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NCoR2 Antibody — Published figure using NCoR2 polyclonal antibody (Product # PA1-843)

NCoR2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a NCoR2 polyclonal antibody (Product # PA1-843) in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.

NCoR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NCoR2 showing positive staining in the nucleus of paraffin-treated Mouse brain tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a NCoR2 polyclonal antibody (Product # PA1-843) diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:200

Immunofluorescence (IF)

1:100-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

4 µg/mL

Published Applications

Miscellaneous PubMed (MISC)

See 1 publication below

Immunoprecipitation (IP)

See 1 publication below

Western Blot (WB)

See 4 publications below

ChIP assay (ChIP)

See 3 publications below

Immunohistochemistry (IHC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2149135

Product Specific Information

PA1-843 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human and mouse cells.

PA1-843 has been successfully used in Western blot, ICC/IF and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects an ~250 kDa protein representing SMRT from HeLa cell nuclear extracts.

The PA1-843 antigen is His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.

Target Information

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CTG repeat protein 26; N-CoR2; NCOR2; Nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor; Silencing mediator of retinoic acid and thyroid hormone receptor; silencing mediator of retinoid acid and thyroid hormone receptor; SMAP270; SMRT; T3 receptor-associating factor; Thyroid-, retinoic-acid-receptor-associated corepressor; TRAC; TRAC1

Gene Aliases: CTG26; N-CoR; N-CoR2; NCOR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC-1; TRAC1

UniProt ID: (Human) Q9Y618, (Mouse) Q9WU42

Entrez Gene ID: (Human) 9612, (Mouse) 20602

Molecular Function: nucleic acid binding transcription cofactor transcription factor