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Invitrogen
NCoR2 Polyclonal Antibody
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FIGURE: 1 / 20
NCoR2 Antibody (PA1-843) in ICC/IF
Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a NCoR2 polyclonal antibody (Product # PA1-843) in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjug... View More
Product Details
PA1-843
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-843 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human and mouse cells.
PA1-843 has been successfully used in Western blot, ICC/IF and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects an ~250 kDa protein representing SMRT from HeLa cell nuclear extracts.
The PA1-843 antigen is His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.
Target Information
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CTG repeat protein 26; N-CoR2; nuclear receptor corepressor; Nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; Silencing mediator of retinoic acid and thyroid hormone receptor; silencing mediator of retinoid acid and thyroid hormone receptor; SMAP270; SMRT; T3 receptor-associating factor; Thyroid-, retinoic-acid-receptor-associated corepressor; TRAC
Gene Aliases: CTG26; N-CoR; N-CoR2; NCOR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC-1; TRAC1
UniProt ID: (Human) Q9Y618, (Mouse) Q9WU42
Entrez Gene ID: (Human) 9612, (Mouse) 20602
DNA binding
transcription corepressor activity
Notch binding
protein binding
transcription factor binding
ligand-dependent nuclear receptor binding
histone deacetylase binding
protein N-terminus binding
RNA polymerase II regulatory region DNA binding
chromatin binding
protein complex binding
glucocorticoid receptor binding
retinoic acid receptor binding
sequence-specific DNA binding
retinoid X receptor binding
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
cellular lipid metabolic process
regulation of cellular ketone metabolic process by negative regulation of transcription from RNA polymerase II promoter
negative regulation of production of miRNAs involved in gene silencing by miRNA
in utero embryonic development
heart morphogenesis
regulation of transcription, DNA-templated
telencephalon development
cell proliferation in forebrain
forebrain development
glucose homeostasis
negative regulation of fat cell differentiation
negative regulation of transcription, DNA-templated
white fat cell differentiation
Type I pneumocyte differentiation
positive regulation of protein deacetylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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