Invitrogen

NCoR2 Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

11 Published Figures

13 References

View all (14) NCoR2 antibodies

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NCoR2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a NCoR2 polyclonal antibody (Product # PA1-843) in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.

NCoR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NCoR2 showing positive staining in the nucleus of paraffin-treated Mouse brain tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a NCoR2 polyclonal antibody (Product # PA1-843) diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

NCoR2 Antibody — SMRT and FOXP1 coregulate c-fms expression in THP-1 cells. (A) Effect of siRNAs specific to FOXP1 or SMRT on c-fms mRNA levels as measured by qRT–PCR (±SEM; [*] P < 0.01, [**] P < 0.005). (B,C) qPCR analysis of chromatin-immunoprecipitated SMRT or FOXP1 on untreated (undiff) and PMA-treated (diff) THP-1 cells using specific anti-SMRT and anti-FOXP1 antibodies and oligonucleotides surrounding the FOX-binding site of the c-fms promoter. (D) Agarose gel representation of ChIP analysis in B and C. (E,F) qPCR analysis of chromatin-immunoprecipitated SMRT or FOXP1 using oligonucleotides surrounding the FOX-binding site of the c-fms promoter performed on THP-1 cells lipofected with FOXP1 siRNA. {KD}

NCoR2 Antibody in Immunohistochemistry (IHC) — Published figure using NCoR2 polyclonal antibody (Product # PA1-843) in Immunohistochemistry

NCoR2 Antibody in Western Blot (WB) — Published figure using NCoR2 polyclonal antibody (Product # PA1-843) in Western Blot

NCoR2 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Published figure using NCoR2 polyclonal antibody (Product # PA1-843) in Western Blot and Immunoprecipitation

NCoR2 Antibody in ChIP assay (ChIP) — Figure 2 FOXO3 keeps the Irf7 gene in check a , ChIP-Seq analysis demonstrates FOXO3 binding profile at Irf7 gene promoter in wild type BMMs. Data are representative of two experiments. b , ChIP of FOXO3 from unstimulated wild-type macrophages shows binding of FOXO3 to the promoters of the target genes. FOXO3 recruitment was not observed at control regions lacking FOXO binding sites (-). Data was normalized to IgG (negative control) and represent the average of three independent experiments +- standard error. c , ChIP analysis of histone acetylation, ubiquitination and methylation at Irf7 gene promoter in WT and Foxo3 -null macrophages. Data represent the average of three independent experiments (+- standard error). d , ChIP-Seq analysis demonstrates increased histone H4 acetylation levels in Foxo3 -null cells. Data are representative of two experiments. e , FOXO3, NCOR2 and HDAC3 are present in the ternary complex at Irf7 promoter, as shown by ChIP-ReChIP assays in unstimulated BMMs. Data was compared to IgG and represent the average of three independent experiments +- standard error. f , ChIP analysis of NCOR2 and HDAC3 binding at Irf7 gene promoter in WT and Foxo3 -null macrophages. Data represent the average of three independent experiments (+- standard error). g , ChIP assay demonstrates increased recruitment of IRF7 at Irf7 gene promoter in Foxo3 -null macrophages relative. Data was normalized to IgG and represent the average of three independent experiments +- standard

Product Details

PA1-843

Applications	Tested Dilution	Publications
Western Blot (WB)	4 µg/mL	View 6 publications 6 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:200	-
Immunoprecipitation (IP)	-	View 2 publications 2 publications
ChIP assay (ChIP)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2149135

Product Specific Information

PA1-843 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human and mouse cells.

PA1-843 has been successfully used in Western blot, ICC/IF and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects an ~250 kDa protein representing SMRT from HeLa cell nuclear extracts.

The PA1-843 antigen is His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.

Target Information

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TBL1XR1 Mutations Drive Extranodal Lymphoma by Inducing a Pro-tumorigenic Memory Fate.

Cell

Venturutti L,Teater M,Zhai A,Chadburn A,Babiker L,Kim D,Béguelin W,Lee TC,Kim Y,Chin CR,Yewdell WT,Raught B,Phillip JM,Jiang Y,Staudt LM,Green MR,Chaudhuri J,Elemento O,Farinha P,Weng AP,Nissen MD,Steidl C,Morin RD,Scott DW,Privé GG,Melnick AM

PA1-843 was used in Immunoprecipitation to show that TBL1XR1 mutations skew the humoral immune response toward generating abnormal immature memory B cells (MB), while impairing plasma cell differentiation.

Thu Jul 23 00:00:00 EDT 2020

ARNT2 Tunes Activity-Dependent Gene Expression through NCoR2-Mediated Repression and NPAS4-Mediated Activation.

Neuron

Sharma N,Pollina EA,Nagy MA,Yap EL,DiBiase FA,Hrvatin S,Hu L,Lin C,Greenberg ME

PA1-843 was used in Western Blotting to conclude that the interplay of bHLH-PAS complexes at activity-dependent regulatory elements maintains temporal control of activity-dependent gene expression and scales somatic inhibition with circuit activity.

Wed Apr 17 00:00:00 EDT 2019

Autoregulatory loop of nuclear corepressor 1 expression controls invasion, tumor growth, and metastasis.

Proceedings of the National Academy of Sciences of the United States of America

Martínez-Iglesias OA,Alonso-Merino E,Gómez-Rey S,Velasco-Martín JP,Martín Orozco R,Luengo E,García Martín R,Ibáñez de Cáceres I,Fernández AF,Fraga MF,González-Peramato P,Varona C,Palacios J,Regadera J,Aranda A

PA1-843 was used in western blot to study how nuclear corepressor 1 contributes to cancer

Tue Jan 19 00:00:00 EST 2016

PHD2/3-dependent hydroxylation tunes cardiac response to β-adrenergic stress via phospholamban.

The Journal of clinical investigation

Xie L,Pi X,Townley-Tilson WH,Li N,Wehrens XH,Entman ML,Taffet GE,Mishra A,Peng J,Schisler JC,Meissner G,Patterson C

PA1-843 was used in immunoprecipitation to investigate how PHD2 and PHD3 modulate contractile function.

Wed Jul 01 00:00:00 EDT 2015

PHD2/3-dependent hydroxylation tunes cardiac response to β-adrenergic stress via phospholamban.

The Journal of clinical investigation

Xie L,Pi X,Townley-Tilson WH,Li N,Wehrens XH,Entman ML,Taffet GE,Mishra A,Peng J,Schisler JC,Meissner G,Patterson C

PA1-843 was used in immunoprecipitation to investigate how PHD2 and PHD3 modulate contractile function.

Wed Jul 01 00:00:00 EDT 2015

NCoR1 and SMRT play unique roles in thyroid hormone action in vivo.

Molecular and cellular biology

Shimizu H,Astapova I,Ye F,Bilban M,Cohen RN,Hollenberg AN

Published figure using NCoR2 polyclonal antibody (Product # PA1-843) in Western Blot

Sun Feb 01 00:00:00 EST 2015

NCoR1 and SMRT play unique roles in thyroid hormone action in vivo.

Molecular and cellular biology

Shimizu H,Astapova I,Ye F,Bilban M,Cohen RN,Hollenberg AN

Published figure using NCoR2 polyclonal antibody (Product # PA1-843) in Western Blot

Sun Feb 01 00:00:00 EST 2015

Thyroid hormone signaling in vivo requires a balance between coactivators and corepressors.

Molecular and cellular biology

Vella KR,Ramadoss P,Costa-E-Sousa RH,Astapova I,Ye FD,Holtz KA,Harris JC,Hollenberg AN

PA1-843 was used in western blot to study the role of the coactivator/corepressor balance in thyroid hormone signaling

Thu May 01 00:00:00 EDT 2014

Corepressors (NCoR and SMRT) as well as coactivators are recruited to positively regulated 1α,25-dihydroxyvitamin D3-responsive genes.

The Journal of steroid biochemistry and molecular biology

Meyer MB,Pike JW

PA1-843 was used in ChIP assay to study the recruitment of both co-activators and co-repressors to vitamin D3-responsive genes

Mon Jul 01 00:00:00 EDT 2013

A FOXO3-IRF7 gene regulatory circuit limits inflammatory sequelae of antiviral responses.

Nature

Litvak V,Ratushny AV,Lampano AE,Schmitz F,Huang AC,Raman A,Rust AG,Bergthaler A,Aitchison JD,Aderem A

PA1-843 was used in ChIP assay to study the role of FOXO3 and IRF7 in minimising inflammatory damage in response to viral infection

Thu Oct 18 00:00:00 EDT 2012

A downstream intergenic cluster of regulatory enhancers contributes to the induction of CYP24A1 expression by 1alpha,25-dihydroxyvitamin D3.

The Journal of biological chemistry

Meyer MB,Goetsch PD,Pike JW

Published figure using NCoR2 polyclonal antibody (Product # PA1-843) in ChIP assay

Fri May 14 00:00:00 EDT 2010

Cooperative regulation in development by SMRT and FOXP1.

Genes & development

Jepsen K,Gleiberman AS,Shi C,Simon DI,Rosenfeld MG

PA1-843 was used in chromatin immunoprecipitation to study the roles of SMRT and FOXP1 in developmental regulation.

Sat Mar 15 00:00:00 EDT 2008

Cooperative regulation in development by SMRT and FOXP1.

Genes & development

Jepsen K,Gleiberman AS,Shi C,Simon DI,Rosenfeld MG

PA1-843 was used in chromatin immunoprecipitation to study the roles of SMRT and FOXP1 in developmental regulation.

Sat Mar 15 00:00:00 EDT 2008

Ataxin 1, a SCA1 neurodegenerative disorder protein, is functionally linked to the silencing mediator of retinoid and thyroid hormone receptors.

Proceedings of the National Academy of Sciences of the United States of America

Tsai CC,Kao HY,Mitzutani A,Banayo E,Rajan H,McKeown M,Evans RM

Published figure using NCoR2 polyclonal antibody (Product # PA1-843) in Western Blot

Tue Mar 23 00:00:00 EST 2004

Bioinformatics

Protein Aliases: CTG repeat protein 26; N-CoR2; nuclear receptor corepressor; Nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; Silencing mediator of retinoic acid and thyroid hormone receptor; silencing mediator of retinoid acid and thyroid hormone receptor; SMAP270; SMRT; T3 receptor-associating factor; Thyroid-, retinoic-acid-receptor-associated corepressor; TRAC

Gene Aliases: CTG26; N-CoR; N-CoR2; NCOR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC-1; TRAC1

UniProt ID: (Human) Q9Y618, (Mouse) Q9WU42

Entrez Gene ID: (Human) 9612, (Mouse) 20602

Molecular Function: chromatin/chromatin-binding, or -regulatory protein