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FIGURE: 1 / 2
NCoR2 Antibody (PA5-141074) in WB
Western blot analysis of NCOR2/SMRT in different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Samples were incubated in NCOR2/SMRT Polyclonal antibody (Product # PA5-141074) using a dilution of 1:1,000 in 5% BSA at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:200,000 for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate; Lane 2: Rat brain tissue lysate.
Product Details
PA5-141074
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within N-terminal human NCOR2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2932526
Target Information
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTG repeat protein 26; N-CoR2; nuclear receptor corepressor; Nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; Silencing mediator of retinoic acid and thyroid hormone receptor; silencing mediator of retinoid acid and thyroid hormone receptor; SMAP270; SMRT; T3 receptor-associating factor; Thyroid-, retinoic-acid-receptor-associated corepressor; TRAC
Gene Aliases: CTG26; N-CoR; N-CoR2; NCOR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC-1; TRAC1
UniProt ID: (Human) Q9Y618, (Mouse) Q9WU42
Entrez Gene ID: (Human) 9612, (Mouse) 20602, (Rat) 360801
DNA binding
transcription corepressor activity
Notch binding
protein binding
transcription factor binding
ligand-dependent nuclear receptor binding
histone deacetylase binding
protein N-terminus binding
RNA polymerase II regulatory region DNA binding
chromatin binding
protein complex binding
glucocorticoid receptor binding
retinoic acid receptor binding
sequence-specific DNA binding
retinoid X receptor binding
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
cellular lipid metabolic process
regulation of cellular ketone metabolic process by negative regulation of transcription from RNA polymerase II promoter
negative regulation of production of miRNAs involved in gene silencing by miRNA
in utero embryonic development
heart morphogenesis
regulation of transcription, DNA-templated
telencephalon development
cell proliferation in forebrain
forebrain development
glucose homeostasis
negative regulation of fat cell differentiation
negative regulation of transcription, DNA-templated
white fat cell differentiation
Type I pneumocyte differentiation
positive regulation of protein deacetylation
lactation
response to organonitrogen compound
response to estradiol
estrous cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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