Invitrogen

NDE1 Polyclonal Antibody

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NDE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using NDE1 Polyclonal antibody (Product # PA5-96583) at a dilution of 1:100 (40x lens).

NDE1 Antibody in Western Blot (WB) — Western blot analysis of extracts of various cell lines, using NDE1 Polyclonal antibody (Product # PA5-96583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:200

Western Blot (WB)

1:500 - 1:2000

Product Specifications

Species Reactivity

Rat, Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1.

Conjugate

Unconjugated

Form

Liquid

Concentration

3.54 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL; Positive Samples: HeLa, HepG2, SKOV3, MCF7, A673, Mouse heart, Mouse lung; Cellular Location: Chromosome, Cleavage furrow, Cytoplasm, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle

Target Information

TFG protein, a recently identified novel protein, is a human homolog of mouse TFG protein. It contains an N-terminal coiled-coil structure, a SPYGQ-rich region followed by octicosapeptide repeats. Research shows that TFG can modulate SHP-1 activity and is a novel member of the NF-kB pathway. The physiological functions of this protein remain largely elusive, but TFG is thought to function by associating with other proteins, including TANK and NEMO. TFG was first identified as a partner of NTRK1 and is involved in generating the thyroid TRK-T3 oncogene, as well as oncogenic rearrangements with ALK in anaplastic lymphoma and NOR1 in myxoid chondrosarcoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: LIS1-interacting protein NUDE1; LIS1-interacting protein NUDE1, rat homolog; nuclear distribution gene E homolog 1; Nuclear distribution protein nudE homolog 1; NudE; nudE nuclear distribution E homolog 1; nudE nuclear distribution gene E homolog 1; rNudE

Gene Aliases: 2810027M15Rik; AU042936; AW822251; HOM-TES-87; LIS4; MHAC; mNudE; NDE; NDE1; NUDE; NUDE1

UniProt ID: (Human) Q9NXR1, (Rat) Q9ES39, (Mouse) Q9CZA6

Entrez Gene ID: (Human) 54820, (Rat) 83836, (Mouse) 67203

Function(s)