Invitrogen

NDE1 Polyclonal Antibody

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NDE1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of NDE1 in paraffin-embedded human breast cancer using NDE1 Polyclonal Antibody (Product # PA5-96583) at a dilution of 1:200.

NDE1 Antibody in Western Blot (WB) — Western Blot analysis of NDE1 in extracts of various cell lines using NDE1 Polyclonal Antibody (Product # PA5-96583) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:200

Western Blot (WB)

1:500 - 1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1.

Conjugate

Unconjugated

Form

Liquid

Concentration

3.54 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2808385

Product Specific Information

Immunogen sequence: MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL; Positive Samples: HeLa, HepG2, SKOV3, MCF7, A673, Mouse heart, Mouse lung; Cellular Location: Chromosome, Cleavage furrow, Cytoplasm, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle

Target Information

NDE1 is s a member of the nuclear distribution E (NudE) family of proteins. It is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. NDE1 protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations to the gene lead to Lissencephaly 4 (LIS4) and Microhydranencephaly (MHAC).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: LIS1-interacting protein NUDE1; LIS1-interacting protein NUDE1, rat homolog; nuclear distribution gene E homolog 1; Nuclear distribution protein nudE homolog 1; NudE; nudE nuclear distribution E homolog 1; nudE nuclear distribution gene E homolog 1; rNudE

Gene Aliases: 2810027M15Rik; AU042936; AW822251; HOM-TES-87; LIS4; MHAC; mNudE; NDE; NDE1; NUDE; NUDE1

UniProt ID: (Human) Q9NXR1, (Rat) Q9ES39, (Mouse) Q9CZA6

Entrez Gene ID: (Human) 54820, (Rat) 83836, (Mouse) 67203

Function(s)