Invitrogen

NDRG1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

17 Published Figures

12 References

View all (24) NDRG1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Tech Support

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NDRG1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CAP43 was performed using 70% confluent log phase HeLa cells treated with 50 ng of TNF-alpha for 20 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CAP43 Rabbit Polyclonal Antibody (Product # 42-6200) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

NDRG1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK 293 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), Mouse Brain (tissue extract) (Lane 4), COLO 205 (Lane 5), HT-29 (Lane 6) and MDA-MB-231 (Lane 7). The blot was probed with Anti-CAP43 Rabbit Polyclonal Antibody (Product # 42-6200, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 43 kDa band corresponding to CAP43 was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

NDRG1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Published figure using NDRG1 polyclonal antibody (Product # 42-6200) in Western Blot and Immunocytochemistry

NDRG1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in NDRG1 KO cell line compared to control cell line using Anti-NDRG1 Polyclonal Antibody (Product # 42-6200). {KO}

Product Details

42-6200

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	View 10 publications 10 publications
Immunocytochemistry (ICC/IF)	2 µg/mL	View 2 publications 2 publications
Immunoprecipitation (IP)	5 µg	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide derived from the N-terminal region of the human and predicted chimpanzee CAP43 proteins, which differs from predicted bovine by one amino acid change and from mouse and rat by two amino acid changes
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Storage conditions	-20°C
RRID	AB_2533532

Target Information

NDRG1 is a 394 amino acid cytoplasmic protein belonging to the NDRG family. It interacts with APO A-I and A-II and plays a role in HDL-mediated cholesterol transport, stress responses, hormone responses, cell growth and differentiation. Its defect causes Charcot-Marie-Tooth disease type 4D (CMT4D) that is characterized by reduced nerve conduction velocities, segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes and hollow feet. NDRG1 plays a growth inhibitory role and serves as a marker of differentiation. It is expressed in placental membranes, prostate, kidney, small intestine, ovary tissues, colon and the cells that border the lumen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mucosa-Associated Lymphoid Tissue 1 Is an Oncogene Inducing Cell Proliferation, Invasion, and Tumor Growth via the Upregulation of NF-κB Activity in Human Prostate Carcinoma Cells.

Biomedicines

Tsui KH,Chang KS,Sung HC,Hsu SY,Lin YH,Hou CP,Yang PS,Chen CL,Feng TH,Juang HH

Published figure using NDRG1 polyclonal antibody (Product # 42-6200) in Western Blot

Wed Mar 03 00:00:00 EST 2021

The Iron Chelator, Dp44mT, Effectively Inhibits Human Oral Squamous Cell Carcinoma Cell Growth in Vitro and in Vivo.

International journal of molecular sciences

Lee JC,Chiang KC,Feng TH,Chen YJ,Chuang ST,Tsui KH,Chung LC,Juang HH

42-6200 was used in western blot determine the effects of iron chelators on oral squamous cell carcinoma cells

Wed Aug 31 00:00:00 EDT 2016

Prostate-derived ets factor represses tumorigenesis and modulates epithelial-to-mesenchymal transition in bladder carcinoma cells.

Cancer letters

Tsui KH,Lin YH,Chung LC,Chuang ST,Feng TH,Chiang KC,Chang PL,Yeh CJ,Juang HH

42-6200 was used in western blot to analyze repression of tumorigenesis and modulation of epithelial-to-mesenchymal transition in bladder carcinoma cells by prostate-derived ets factor

Sat May 28 00:00:00 EDT 2016

Growth differentiation factor-15: a p53- and demethylation-upregulating gene represses cell proliferation, invasion, and tumorigenesis in bladder carcinoma cells.

Scientific reports

Tsui KH,Hsu SY,Chung LC,Lin YH,Feng TH,Lee TY,Chang PL,Juang HH

42-6200 was used in western blot to analyze growth differentiation factor-15 as a p53- and demehtylation-upregulating gene that represses invasion, cell proliferation, and tumorigenesis in bladder carcinoma cells

Fri Aug 07 00:00:00 EDT 2015

N-myc downstream-regulated gene 1 (NDRG1) mediates pomegranate juice protection from apoptosis in hypoxic BeWo cells but not in primary human trophoblasts.

Placenta

Chen B,Zaveri PG,Longtine MS,Nelson DM

42-6200 was used in immunocytochemistry to test if pomegranate juice protects trophoblasts from hypoxia-induced apoptosis by modulating NDRG1 expression.

Sat Aug 01 00:00:00 EDT 2015

N-myc downstream-regulated gene 1 (NDRG1) mediates pomegranate juice protection from apoptosis in hypoxic BeWo cells but not in primary human trophoblasts.

Placenta

Chen B,Zaveri PG,Longtine MS,Nelson DM

42-6200 was used in immunocytochemistry to test if pomegranate juice protects trophoblasts from hypoxia-induced apoptosis by modulating NDRG1 expression.

Sat Aug 01 00:00:00 EDT 2015

N-myc downstream-regulated gene 1 downregulates cell proliferation, invasiveness, and tumorigenesis in human oral squamous cell carcinoma.

Cancer letters

Lee JC,Chung LC,Chen YJ,Feng TH,Juang HH

42-6200 was used in immunocytochemistry and western blot to determine the role and function of NDRG1 in oral squamous cell carcinoma

Sun Dec 28 00:00:00 EST 2014

Mechanisms by which interleukin-6 attenuates cell invasion and tumorigenesis in human bladder carcinoma cells.

BioMed research international

Tsui KH,Wang SW,Chung LC,Feng TH,Lee TY,Chang PL,Juang HH

Published figure using NDRG1 polyclonal antibody (Product # 42-6200) in Western Blot

Wed Dec 04 00:00:00 EST 2013

Metallothionein 3: an androgen-upregulated gene enhances cell invasion and tumorigenesis of prostate carcinoma cells.

The Prostate

Juang HH,Chung LC,Sung HC,Feng TH,Lee YH,Chang PL,Tsui KH

42-6200 was used in western blot to study metallothioneins in prostate carcinoma cells.

Tue Oct 01 00:00:00 EDT 2013

Glycoprotein transmembrane nmb: an androgen-downregulated gene attenuates cell invasion and tumorigenesis in prostate carcinoma cells.

The Prostate

Tsui KH,Chang YL,Feng TH,Chang PL,Juang HH

42-6200 was used in western blot to investigate the function and regulation of glycoprotein transmembrane nmb in the human prostate.

Sat Sep 15 00:00:00 EDT 2012

Upregulation of prostate-derived Ets factor by luteolin causes inhibition of cell proliferation and cell invasion in prostate carcinoma cells.

International journal of cancer

Tsui KH,Chung LC,Feng TH,Chang PL,Juang HH

42-6200 was used in western blot to report the antitumor function of luteolin via upregulation of PDEF gene expression in human prostate carcinoma cells.

Fri Jun 15 00:00:00 EDT 2012

L-Mimosine blocks cell proliferation via upregulation of B-cell translocation gene 2 and N-myc downstream regulated gene 1 in prostate carcinoma cells.

American journal of physiology. Cell physiology

Chung LC,Tsui KH,Feng TH,Lee SL,Chang PL,Juang HH

42-6200 was used in western blot to investigate the molecular mechanisms of L-mimosine on cell cycle modulation in prostate carcinoma cells.

Wed Feb 15 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Differentiation-related gene 1 protein; DRG-1; N-myc downstream regulated 1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Protein Ndr1; Protein NDRG1; protein regulated by oxygen-1; Reducing agents and tunicamycin-responsive protein; Rit42; RTP

Gene Aliases: CAP43; DRG1; Ndr1; NDRG1; Ndrl; RTP; Tdd5

NDRG1 Polyclonal Antibody

Product Details

42-6200

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage conditions

RRID

Target Information

Bioinformatics

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