Invitrogen

NDRG2 Polyclonal Antibody

View all (16) NDRG2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

NDRG2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NDRG2 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NDRG2 polyclonal antibody (Product # PA5-109260), followed by DAPI (blue).

NDRG2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NDRG2 in paraffin-embedded human prostate tissue. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), and followed by hematoxylin.

NDRG2 Antibody in Western Blot (WB) — Western blot analysis of NDRG2 in mouse brain (1) and mouse skeletal muscle (2) tissue lysate. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), at a dilution of 1:1000.

NDRG2 Antibody in Flow Cytometry (Flow) — Flow cytometry of NDRG2 in HepG2 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Product Details

PA5-109260

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within Human NDRG2 aa 13-160

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

RRID

AB_2854671

Product Specific Information

Positive Control: Mouse brain tissue, mouse skeletal muscle tissue, HepG2, LOVO, SH-SY-5Y, rat brain tissue, human liver tissue, human prostate tissue.

Target Information

NDRG2's gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG2 is a cytoplasmic protein that may play a role in neurite outgrowth. Its gene may be involved in glioblastoma carcinogenesis.This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cytoplasmic protein Ndr1; DKFZp781G1938; FLJ25522; N-myc downstream regulator 2; N-myc downstream-regulated gene 2 protein; NDR1-related protein NDR2; NDRG2var; Protein NDRG2; Protein Syld709613; syld709613 protein

Gene Aliases: KIAA1248; NDRG2; SYLD

UniProt ID: (Human) Q68DW2

Entrez Gene ID: (Human) 57447

Molecular Function: serine protease

Function(s)

negative regulation of cytokine production signal transduction regulation of vascular endothelial growth factor production Wnt signaling pathway substantia nigra development cell differentiation negative regulation of smooth muscle cell proliferation negative regulation of ERK1 and ERK2 cascade regulation of platelet-derived growth factor production

Process(es)

molecular_function protein binding