Invitrogen

NDRG2 Polyclonal Antibody

Product Image Gallery

NDRG2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NDRG2 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NDRG2 polyclonal antibody (Product # PA5-109260), followed by DAPI (blue).

NDRG2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NDRG2 in paraffin-embedded human prostate tissue. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), and followed by hematoxylin.

NDRG2 Antibody in Western Blot (WB) — Western blot analysis of NDRG2 in mouse brain (1) and mouse skeletal muscle (2) tissue lysate. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), at a dilution of 1:1000.

NDRG2 Antibody in Flow Cytometry (Flow) — Flow cytometry of NDRG2 in HepG2 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Product Details

PA5-109260

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein within Human NDRG2 aa 13-160 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2854671

Product Specific Information

Positive Control: Mouse brain tissue, mouse skeletal muscle tissue, HepG2, LOVO, SH-SY-5Y, rat brain tissue, human liver tissue, human prostate tissue.

Target Information

NDRG2's gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG2 is a cytoplasmic protein that may play a role in neurite outgrowth. Its gene may be involved in glioblastoma carcinogenesis.This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cytoplasmic protein Ndr1; DKFZp781G1938; FLJ25522; N-myc downstream regulator 2; N-myc downstream-regulated gene 2 protein; NDR1-related protein NDR2; NDRG2var; Protein NDRG2; Protein Syld709613; syld709613 protein

Gene Aliases: KIAA1248; NDRG2; SYLD

UniProt ID: (Human) Q9UN36

Entrez Gene ID: (Human) 57447

Molecular Function: serine protease