Invitrogen

NDUFAF2 Polyclonal Antibody

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NDUFAF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of NDUFAF2 in paraffin embedded human small intestine tissue using a NDUFAF2 polyclonal antibody (Product # PA5-98667) at a dilution of 1:500. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

NDUFAF2 Antibody in Western Blot (WB) — Western Blot analysis of NDUFAF2 using a NDUFAF2 Polyclonal antibody (Product # PA5-98667) at a concentration of 2.7 µg/mL. Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 20 kDa.

Product Details

PA5-98667

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500-1:1,000	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 protein (117-169AA) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	2.78 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2813280

Target Information

Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-kappa-B-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B17.2-like; B17.2L; B172-like; B172L; Mimitin; MMTN; Myc-induced mitochondrial protein; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2; NADH dehydrogenase (ubiquinone) complex I, assembly factor 2; NADH dehydrogenase 1 alpha subcomplex assembly factor 2; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2; NDUFA12-like protein

Gene Aliases: B17.2L; mimitin; MMTN; NDUFA12L; NDUFAF2

UniProt ID: (Human) Q8N183

Entrez Gene ID: (Human) 91942

Molecular Function: chaperone