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Invitrogen
NDUFB9 Polyclonal Antibody
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FIGURE: 1 / 6
NDUFB9 Antibody (PA5-21668) in ICC/IF
Immunofluorescence analysis of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NDUFB9 Polyclonal Antibody (Product # PA5-21668) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Seconda... View More
Product Details
PA5-21668
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human NDUFB9. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLaS3, HepG2, mouse brain.
Predicted reactivity: Mouse (86%), Rat (86%), Chimpanzee (99%), Bovine (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
LYRM3, also known as NADH dehydrogenase (ubiquinone) 1 beta subcomplex 9 (NDUFB9), is a ubiquitously expressed LYR-motif containing protein. It has been suggested to be a candidate gene for the branchio-oto-renal (BOR) syndrome, which is characterized by branchial and renal abnormalities and heredity deafness disorders. Other than its LYR-motif, LYRM3 appears to have no functional or structural relationship to either LYRM1 or LYRM2.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CI-B22; complex I B22 subunit; complex I subunit; Complex I-B22; LYR motif-containing protein 3; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; NADH-ubiquinone oxidoreductase B22 subunit; unnamed protein product
Gene Aliases: 1190008J14Rik; B22; CI-B22; LYRM3; MC1DN24; NDUFB9; UQOR22
UniProt ID: (Human) Q9Y6M9, (Mouse) Q9CQJ8
Entrez Gene ID: (Human) 4715, (Mouse) 66218, (Rat) 299954
mitochondrial electron transport, NADH to ubiquinone
sensory perception of sound
aerobic respiration
mitochondrial ATP synthesis coupled proton transport
hydrogen ion transmembrane transport
transport
oxidation-reduction process
mitophagy in response to mitochondrial depolarization
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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