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|Tested species reactivity||Bovine, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Whole mitochondria from Human Heart|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/ml|
|Immunocytochemistry (ICC)||5-10 ug/ml|
|Immunofluorescence (IF)||5-10 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Use Isolated mitochondria from Human, Bovine Rat and Mouse hearts; Cultured Human fibroblasts; HeLa cells; Human colon and cerebellum tissues as positive control.
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cysteine dietary supplementation reverses the decrease in mitochondrial ROS production at complex I induced by methionine restriction.
459130 was used in western blot to investigate cysteine and methionine dietary supplementation in aging rodents.
|Gomez A,Gomez J,Lopez Torres M,Naudi A,Mota-Martorell N,Pamplona R,Barja G||Journal of bioenergetics and biomembranes (47:199)||2015|
Independent and additive effects of atenolol and methionine restriction on lowering rat heart mitochondria oxidative stress.
459130 was used in western blot to study the effects on rat cardiac mitochondrial oxidative stress of simultaneous treatment with atenolol plus methionine restriction.
|Sanchez-Roman I,Gomez A,Naudí A,Jove M,Gómez J,Lopez-Torres M,Pamplona R,Barja G||Journal of bioenergetics and biomembranes (46:159)||2014|
CI-30; CI-30kD; complex I 30kDa subunit; Complex I-30kD; mitochondrial; NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 3; NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; NADH dehydrogenase-ubiquinone 30 kDa subunit; NADH-ubiquinone oxidoreductase 30 kDa subunit
0610010M09Rik; BOS_15116; CI-30; CI-30kD; NDUFS3