|Tested species reactivity||Bovine, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Whole mitochondria from Human Heart|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/ml|
|Immunocytochemistry (ICC)||5-10 ug/ml|
|Immunofluorescence (IF)||5-10 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Use Isolated mitochondria from Human, Bovine Rat and Mouse hearts; Cultured Human fibroblasts; HeLa cells; Human colon and cerebellum tissues as positive control.
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Calsequestrins in skeletal and cardiac muscle from adult Danio rerio.
459130 was used in western blot to analyze Danio rerio for calsequestrins in cardiac and skeletal muscle
|Furlan S,Mosole S,Murgia M,Nagaraj N,Argenton F,Volpe P,Nori A||Journal of muscle research and cell motility (37:27)||2016|
Cysteine dietary supplementation reverses the decrease in mitochondrial ROS production at complex I induced by methionine restriction.
459130 was used in western blot to investigate cysteine and methionine dietary supplementation in aging rodents.
|Gomez A,Gomez J,Lopez Torres M,Naudi A,Mota-Martorell N,Pamplona R,Barja G||Journal of bioenergetics and biomembranes (47:199)||2015|
Independent and additive effects of atenolol and methionine restriction on lowering rat heart mitochondria oxidative stress.
459130 was used in western blot to study the effects on rat cardiac mitochondrial oxidative stress of simultaneous treatment with atenolol plus methionine restriction.
|Sanchez-Roman I,Gomez A,Naudí A,Jove M,Gómez J,Lopez-Torres M,Pamplona R,Barja G||Journal of bioenergetics and biomembranes (46:159)||2014|
Insulin and IGF-1 improve mitochondrial function in a PI-3K/Akt-dependent manner and reduce mitochondrial generation of reactive oxygen species in Huntington's disease knock-in striatal cells.
459130 was used in western blot to examine the role of insulin and IGF-1 on the mitochondria of striatal cells derived from HD knock-in mice.
|Ribeiro M,Rosenstock TR,Oliveira AM,Oliveira CR,Rego AC||Free radical biology and medicine (74:129)||2014|