|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 175 of NDUFS4 (Uniprot ID#O43181)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-21677 targets NDUFS4 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human, rat, and mouse samples.
The PA5-21677 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 175 (O43181) of NDUFS4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Activation of mTORC1 is essential for ß-adrenergic stimulation of adipose browning.
PA5-21677 was used in western blot describe a signaling pathway from beta-adrenergic receptor and protein kinase A via mTORC1 that is required for adipose browning by catecholamines
|Liu D,Bordicchia M,Zhang C,Fang H,Wei W,Li JL,Guilherme A,Guntur K,Czech MP,Collins S||The Journal of clinical investigation (126:1704)||2016|