Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2400 | View 6 publications 6 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Non-human primate, Rat |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
NEDD4 Fusion Protein Ag16291 (970-1319 aa encoded by BC152452) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.22 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
This antibody can recognize NEDD4-1 and NEDD4-2.
Immunogen sequence: TVLEDSYRR IMGVKRADFL KARLWIEFDG EKGLDYGGVA REWFFLISKE MFNPYYGLFE YSATDNYTLQ INPNSGLCNE DHLSYFKFIG RVAGMAVYHG KLLDGFFIRP FYKMMLHKPI TLHDMESVDS EYYNSLRWIL ENDPTELDLR FIIDEELFGQ THQHELKNGG SEIVVTNKNK KEYIYLVIQW RFVNRIQKQM AAFKEGFFEL IPQDLIKIFD ENELELLMCG LGDVDVNDWR EHTKYKNGYS ANHQVIQWFW KAVLMMDSEK RIRLLQFVTG TSRVPMNGFA ELYGSNGPQS FTVEQWGTPE KLPRAHTCFN RLDLPPYESF EELWDKLQMA IENTQGFDGV D (970-1319 aa encoded by BC152452)
The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated gene 4; neural precursor cell expressed, developmentally down-regulated gene 4A; neural precursor cell expressed, developmentally down-regulted gene 4; OTTHUMP00000217840; OTTHUMP00000217841; OTTHUMP00000217842; receptor-potentiating factor 1
Gene Aliases: AA959633; AL023035; AU019897; E430025J12Rik; KIAA0093; mKIAA0093; Nedd-4; NEDD4; NEDD4-1; Nedd4a; PIG53; RPF1
UniProt ID: (Human) B7ZM60, (Rat) Q62940, (Mouse) P46935
Entrez Gene ID: (Human) 4734, (Rat) 25489, (Mouse) 17999
Molecular Function:
ubiquitin-protein ligase
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