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Proteintech

NEDD4 Polyclonal Antibody

6 References
View all (31) NEDD4 antibodies
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

21698-1-AP

Applications Tested Dilution Publications

Western Blot (WB)

 1:500-1:2400 View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

 1:20-1:200 -

Immunoprecipitation (IP)

 0.5-4.0 µg -
Product Specifications

Species Reactivity

 Human, Mouse, Non-human primate, Rat

Published species

 Human, Mouse, Rat

Host/Isotype

 Rabbit / IgG

Class

 Polyclonal

Type

 Antibody

Immunogen

NEDD4 Fusion Protein Ag16291 (970-1319 aa encoded by BC152452)
View immunogen

Conjugate

 Unconjugated

Form

 Liquid

Concentration

 0.22 mg/mL

Purification

 Antigen affinity chromatography

Storage buffer

 PBS, pH 7.3, with 50% glycerol

Contains

 0.02% sodium azide

Storage conditions

 -20°C

Product Specific Information

This antibody can recognize NEDD4-1 and NEDD4-2.

Immunogen sequence: TVLEDSYRR IMGVKRADFL KARLWIEFDG EKGLDYGGVA REWFFLISKE MFNPYYGLFE YSATDNYTLQ INPNSGLCNE DHLSYFKFIG RVAGMAVYHG KLLDGFFIRP FYKMMLHKPI TLHDMESVDS EYYNSLRWIL ENDPTELDLR FIIDEELFGQ THQHELKNGG SEIVVTNKNK KEYIYLVIQW RFVNRIQKQM AAFKEGFFEL IPQDLIKIFD ENELELLMCG LGDVDVNDWR EHTKYKNGYS ANHQVIQWFW KAVLMMDSEK RIRLLQFVTG TSRVPMNGFA ELYGSNGPQS FTVEQWGTPE KLPRAHTCFN RLDLPPYESF EELWDKLQMA IENTQGFDGV D (970-1319 aa encoded by BC152452)

Target Information

The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated gene 4; neural precursor cell expressed, developmentally down-regulated gene 4A; neural precursor cell expressed, developmentally down-regulted gene 4; OTTHUMP00000217840; OTTHUMP00000217841; OTTHUMP00000217842; receptor-potentiating factor 1

Gene Aliases: AA959633; AL023035; AU019897; E430025J12Rik; KIAA0093; mKIAA0093; Nedd-4; NEDD4; NEDD4-1; Nedd4a; PIG53; RPF1

UniProt ID: (Human) B7ZM60, (Rat) Q62940, (Mouse) P46935

Entrez Gene ID: (Human) 4734, (Rat) 25489, (Mouse) 17999

Molecular Function: ubiquitin-protein ligase

Function(s)
adaptive immune response outflow tract morphogenesis endocardial cushion development protein monoubiquitination protein targeting to lysosome lysosomal transport neuromuscular junction development negative regulation of sodium ion transport negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage positive regulation of phosphatidylinositol 3-kinase signaling response to denervation involved in regulation of muscle adaptation regulation of macroautophagy protein ubiquitination transmission of virus negative regulation of vascular endothelial growth factor receptor signaling pathway neuron projection development receptor internalization receptor catabolic process cellular response to UV regulation of ion transmembrane transport T cell activation regulation of membrane potential protein ubiquitination involved in ubiquitin-dependent protein catabolic process glucocorticoid receptor signaling pathway ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway development involved in symbiotic interaction positive regulation of protein catabolic process positive regulation of nucleocytoplasmic transport blood vessel morphogenesis regulation of dendrite morphogenesis regulation of synapse organization progesterone receptor signaling pathway response to calcium ion protein K63-linked ubiquitination regulation of potassium ion transmembrane transporter activity negative regulation of sodium ion transmembrane transporter activity negative regulation of transcription from RNA polymerase II promoter immune response nervous system development
Process(es)
protein binding protein C-terminus binding ligase activity sodium channel inhibitor activity protein domain specific binding beta-2 adrenergic receptor binding ubiquitin binding phosphoserine binding phosphothreonine binding ubiquitin protein ligase activity RNA polymerase binding proline-rich region binding ubiquitin-protein transferase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
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