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NEDD4 Polyclonal Antibody

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NEDD4 Polyclonal Antibody

Product Details

BS-7877R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Flow Cytometry (Flow)

1:20-1:100
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human NEDD4, amino acids 801-900.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; KIAA0093; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase; neural precursor cell expressed, developmentally down-regulated gene 4; neural precursor cell expressed, developmentally down-regulated gene 4A; neural precursor cell expressed, developmentally down-regulted gene 4; OTTHUMP00000217840; OTTHUMP00000217841; OTTHUMP00000217842; PIG53; receptor-potentiating factor 1; unnamed protein product

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Gene Aliases: AA959633; AL023035; AU019897; E430025J12Rik; KIAA0093; mKIAA0093; Nedd-4; NEDD4; NEDD4-1; Nedd4a; PIG53; RPF1

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UniProt ID: (Human) P46934, (Rat) Q62940, (Mouse) P46935

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Entrez Gene ID: (Human) 4734, (Rat) 25489, (Mouse) 17999

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Function(s)
ubiquitin-protein transferase activity protein binding channel inhibitor activity transferase activity potassium channel inhibitor activity sodium channel inhibitor activity enzyme binding protein domain specific binding beta-2 adrenergic receptor binding ionotropic glutamate receptor binding ubiquitin binding ion channel binding phosphoserine binding phosphothreonine binding ubiquitin protein ligase activity RNA polymerase binding proline-rich region binding protein C-terminus binding ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase
Process(es)
negative regulation of transcription from RNA polymerase II promoter adaptive immune response outflow tract morphogenesis endocardial cushion development transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process protein monoubiquitination protein targeting to lysosome sodium ion transport immune response cellular response to DNA damage stimulus lysosomal transport nervous system development neuromuscular junction development regulation of cell communication negative regulation of sodium ion transport regulation of macroautophagy protein ubiquitination regulation of signaling negative regulation of vascular endothelial growth factor receptor signaling pathway neuron projection development receptor internalization receptor catabolic process cellular response to UV T cell activation regulation of protein catabolic process regulation of membrane potential glucocorticoid receptor signaling pathway ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway development involved in symbiotic interaction innate immune response positive regulation of protein catabolic process viral budding positive regulation of nucleocytoplasmic transport blood vessel morphogenesis regulation of dendrite morphogenesis regulation of synapse organization progesterone receptor signaling pathway response to calcium ion establishment of localization in cell positive regulation of protein kinase B signaling regulation of biological quality protein K63-linked ubiquitination negative regulation of potassium ion export across plasma membrane negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage positive regulation of phosphatidylinositol 3-kinase signaling response to denervation involved in regulation of muscle adaptation transmission of virus regulation of ion transmembrane transport protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of potassium ion transmembrane transporter activity
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