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Invitrogen
NEDD4L Polyclonal Antibody
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FIGURE: 1 / 2
NEDD4L Antibody (PA5-98752) in ICC/IF
Immunofluorescent analysis of NEDD4L in HepG2 cells using a NEDD4L polyclonal antibody (Product # PA5-98752) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Product Details
PA5-98752
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human E3 ubiquitin-protein ligase NEDD4-like protein (289-494AA)
Conjugate
Form
Liquid
Concentration
2.51 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alternatively spliced; amino-terminal variant; developmentally down-regulated 4-like; E3 ubiquitin-protein ligase NEDD4-like; FLJ33870; HECT-type E3 ubiquitin transferase NED4L; KIAA0439; Nedd4-2; NEDD4.2; neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase; similar to NEDD4; ubiquitin-ligase; neural precursor cell expressed, developmentally down-regulated 4-like protein; ubiquitin-protein ligase Rsp5; unnamed protein product
Gene Aliases: hNEDD4-2; KIAA0439; NEDD4-2; NEDD4.2; NEDD4L; NEDL3; PVNH7; RSP5
UniProt ID: (Human) Q96PU5
Entrez Gene ID: (Human) 23327
regulation of membrane depolarization
ubiquitin-dependent protein catabolic process
protein monoubiquitination
ion transport
neuromuscular junction development
protein ubiquitination
cell differentiation
neuron projection development
receptor internalization
regulation of protein stability
receptor catabolic process
ion transmembrane transport
regulation of membrane potential
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of protein catabolic process
regulation of dendrite morphogenesis
regulation of synapse organization
regulation of membrane repolarization
regulation of biological quality
protein K48-linked ubiquitination
ventricular cardiac muscle cell action potential
sodium ion import across plasma membrane
negative regulation of potassium ion transmembrane transport
regulation of sodium ion transmembrane transport
negative regulation of sodium ion transmembrane transport
negative regulation of potassium ion export across plasma membrane
negative regulation of sodium ion import across plasma membrane
positive regulation of dendrite extension
negative regulation of protein localization to cell surface
positive regulation of caveolin-mediated endocytosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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