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Invitrogen
NEDD8 Polyclonal Antibody
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FIGURE: 1 / 19
NEDD8 Antibody (34-1400) in WB
Nature communications 2023 -
Fig. 6 NLRX1-mediated ROS controls NF-kappaB signalling and affects cullin 1 NEDDylation. Immunofluorescence microscopy of A549 cells stained with antibody for the p65 NF-kappaB subunit. Cells were pre-treated with mitoTEMPO (10 muM, 2 h pre-infection), or with vehicle control (DMSO) and infected with Kp52145 for 3 h ( a ). b Cells were transfected with NLRX1 siRNA (50 nM) or a non-silencing control (AS) an... View More
Product Details
34-1400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Insect
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the N-terminal sequence of human NEDD8
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: NEDD-8; NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Ubiquitin-like protein NEDD8
Gene Aliases: NEDD-8; NEDD8
UniProt ID: (Human) Q15843
Entrez Gene ID: (Human) 4738
regulation of transcription from RNA polymerase II promoter
proteolysis
ubiquitin-dependent protein catabolic process
protein localization
anatomical structure morphogenesis
protein ubiquitination
modification-dependent protein catabolic process
regulation of proteolysis
protein modification process
protein neddylation
cellular response to camptothecin
regulation of postsynapse assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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