|Tested species reactivity||Human|
|Published species reactivity||Insect, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal sequence of human NEDD8|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
COP9-Signalosome deneddylase activity is enhanced by simultaneous neddylation: insights into the regulation of an enzymatic protein complex.
34-1400 was used in western blot to study the effect of neddylation on COP9-Signalosome deneddylase activity
|Bornstein G,Grossman C||Cell division (10:null)||2015|
The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics.
34-1400 was used in western blot to elucidate how cycle inhibiting factor modulates NEDD8-dependent signaling.
|Toro TB,Toth JI,Petroski MD||The Journal of biological chemistry (288:14716)||2013|
|Insect||Not Cited||UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1¿ accumulation.||Bandau S,Knebel A,Gage ZO,Wood NT,Alexandru G||BMC biology (10:null)||2012|
A gatekeeper residue for NEDD8-activating enzyme inhibition by MLN4924.
34-1400 was used in western blot to elucidate resistance to MLN4924.
|Toth JI,Yang L,Dahl R,Petroski MD||Cell reports (1:309)||2012|
Development and validation of a method for profiling post-translational modification activities using protein microarrays.
34-1400 was used in western blot to generate a protein microarray-based assay to measure post-translational modifications
|Del Rincón SV,Rogers J,Widschwendter M,Sun D,Sieburg HB,Spruck C||PloS one (5:null)||2010|