Invitrogen

NEDD8 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

16 Published Figures

6 References

View all (20) NEDD8 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (16)
Advanced Verification (2)

FIGURE: 1 / 18

NEDD8 Antibody (34-1400) in WB

Cell division 2015 - Fig. 1 Cul1 deneddylation is mediated specifically by CSN. a 1 nM His-6-Cul1/ROC1 was pre-neddylated for 30 min at 30degC; and for 20 min more, at 30degC, following the addition of elevated volumes of HeLa cells extract. Lanes 5 - 6 represent equivalent volumes of HeLa cells extract blotted with anti-Cul1 antibody; this compares the resultant fraction of endogenic Cul1 with its neddylated conjugate, to the ... View More

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NEDD8 Antibody — Fig. 4 IL-1beta treatment of primary neuronal cultures leads to NEDD8 translocation. Fluorescent immunocytochemistry of representative cultures of untreated ( a ) and IL-1beta-treated ( b ) primary rat neurons ( n = 6 each) depict greater translocation of NEDD8 (green) from nucleus (blue) to cytoplasm when IL-1beta is present, ** p < 0.01, ++ p < 0.01 ( c ). Quantification of western immunoblots ( d ) of nuclear (histone) and cytoplasmic (actin) cell fractions from untreated and IL-1beta-treated cultures ( n = 6 separate experiments) demonstrates the susceptibility of NEDD8 to undergo nuclear to cytoplasmic translocation in the presence of excess IL-1beta, ** p < 0.01, *** p < 0.01 ( e ). Quantification of NEDD8/parkin colocation (<= 40 nm) in untreated NT2 cells ( f ) compared with IL-1beta-treated NT2 cells ( g ) provides evidence of IL-1beta-induced interaction between of parkin and NEDD8 in NT2 cells, two-tailed t test, * p < 0.05 ( h ). NEDD8-parkin proximity (red dots) was quantified with FIJI (ImageJ) Particle Analysis {TM}

Product Details

34-1400

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 5 publications 5 publications

ELISA (ELISA)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Insect

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the N-terminal sequence of human NEDD8

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533155

Target Information

NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

COP9-Signalosome deneddylase activity is enhanced by simultaneous neddylation: insights into the regulation of an enzymatic protein complex.

Cell division

Bornstein G,Grossman C

Published figure using NEDD8 polyclonal antibody (Product # 34-1400) in Western Blot

Wed Aug 12 00:00:00 EDT 2015

The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics.

The Journal of biological chemistry

Toro TB,Toth JI,Petroski MD

Published figure using NEDD8 polyclonal antibody (Product # 34-1400) in Western Blot

Fri May 24 00:00:00 EDT 2013

The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics.

The Journal of biological chemistry

Toro TB,Toth JI,Petroski MD

Published figure using NEDD8 polyclonal antibody (Product # 34-1400) in Western Blot

Fri May 24 00:00:00 EDT 2013

UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1α accumulation.

BMC biology

Bandau S,Knebel A,Gage ZO,Wood NT,Alexandru G

Thu Apr 26 00:00:00 EDT 2012

A gatekeeper residue for NEDD8-activating enzyme inhibition by MLN4924.

Cell reports

Toth JI,Yang L,Dahl R,Petroski MD

34-1400 was used in western blot to elucidate resistance to MLN4924.

Thu Apr 19 00:00:00 EDT 2012

Development and validation of a method for profiling post-translational modification activities using protein microarrays.

PloS one

Del Rincón SV,Rogers J,Widschwendter M,Sun D,Sieburg HB,Spruck C

Published figure using NEDD8 polyclonal antibody (Product # 34-1400) in Western Blot

Mon Jun 28 00:00:00 EDT 2010

Development and validation of a method for profiling post-translational modification activities using protein microarrays.

PloS one

Del Rincón SV,Rogers J,Widschwendter M,Sun D,Sieburg HB,Spruck C

Published figure using NEDD8 polyclonal antibody (Product # 34-1400) in Western Blot

Mon Jun 28 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: NEDD-8; NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; Ubiquitin-like protein Nedd8

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Gene Aliases: NEDD-8; NEDD8

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UniProt ID: (Human) Q15843

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Entrez Gene ID: (Human) 4738

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Function(s)

regulation of transcription from RNA polymerase II promoter cellular protein modification process proteolysis ubiquitin-dependent protein catabolic process transforming growth factor beta receptor signaling pathway protein localization anatomical structure morphogenesis response to organic cyclic compound protein neddylation

Process(es)

protein binding ubiquitin protein ligase binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NEDD8 Polyclonal Antibody

NEDD8 Antibody (34-1400) in WB

Product Details

34-1400

Western Blot (WB)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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