Invitrogen

NEFL Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (74) NEFL antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)
Advanced Verification (1)

FIGURE: 1 / 4

NEFL Antibody (PA1-10000) in WB

Western blot was performed using Anti-NEFL Polyclonal Antibody (Product # PA1-10000) and a 68 kDa band corresponding to NEFL was observed in PC-12 differentiated to neurons, Mouse Cerebellum, Mouse Brain and Rat Brain but not in PC-12, Mouse Heart and Mouse Lung along with few uncharacterized bands (*). Membrane enriched extracts (30 µg lysate) of PC-12 (Lane 1), PC-12 differentiated to neurons (Lane 2), Mouse Cerebellum (Lane 3), Mouse Brain (Lane 4), Rat Brain (Lane 5), Mouse Heart (Lane 6) and Mouse Lung (Lane 7) were electrophoresed us... View More

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NEFL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NEFL in rat cerebellum. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained with an NEFL polyclonal antibody (Product # PA1-10000) at a dilution of 1:2,000 in green, and costained with NeuN/Fox3 monoclonal antibody at a dilution of 1:5,000 in red. The NEFL polyclonal antibody (Product # PA1-10000) antibody labels perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while the NeuN/Fox3 antibody stains the nuclei and proximal cytoplasm of neurons.

NEFL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of NEFL was observed in PC-12 differentiated to neurons in comparison to PC-12 undifferentiated cells. Relative expression was also observed in Mouse Cerebellum, Mouse Brain and Rat Brain in comparison to Mouse Heart and Mouse Lung using Anti-NEFL Polyclonal antibody (Product # PA1-10000) in Western Blot. {RE}

Product Details

PA1-10000

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:5,000

Immunohistochemistry (IHC)

1:2,000

Immunocytochemistry (ICC/IF)

1:2,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Published species

Human

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human NF-L protein

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

RRID

AB_1077181

Product Specific Information

PA1-10000 was made against a preparation of full length human recombinant NF-L protein. It binds NF-L from a variety of species including human, rat and mouse.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enzymatically crosslinked gelatin-laminin hydrogels for applications in neuromuscular tissue engineering.

Biomaterials science

Besser RR,Bowles AC,Alassaf A,Carbonero D,Claure I,Jones E,Reda J,Wubker L,Batchelor W,Ziebarth N,Silvera R,Khan A,Maciel R,Saporta M,Agarwal A

PA1-10000 was used in Immunocytochemistry to report a water-soluble and non-toxic method to incorporate additional extracellular matrix proteins into gelatin hydrogels, while obviating the use of chemical crosslinkers such as glutaraldehyde.

Tue Jan 21 00:00:00 EST 2020

Bioinformatics

Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110

UniProt ID: (Bovine) P02548, (Human) P07196, (Mouse) P08551, (Rat) P19527

Entrez Gene ID: (Bovine) 281348, (Human) 4747, (Pig) 100521224, (Mouse) 18039, (Rat) 83613

Function(s)