Invitrogen

NEFL Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (47) NEFL antibodies

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NEFL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NEFL in rat cerebellum. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained with an NEFL polyclonal antibody (Product # PA1-10000) at a dilution of 1:2,000 in green, and costained with NeuN/Fox3 monoclonal antibody at a dilution of 1:5,000 in red. The NEFL polyclonal antibody (Product # PA1-10000) antibody labels perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while the NeuN/Fox3 antibody stains the nuclei and proximal cytoplasm of neurons.

NEFL Antibody in Western Blot (WB) — Western blot was performed using Anti-NEFL Polyclonal Antibody (Product # PA1-10000) and a 68 kDa band corresponding to NEFL was observed in PC-12 differentiated to neurons, Mouse Cerebellum, Mouse Brain and Rat Brain but not in PC-12, Mouse Heart and Mouse Lung along with few uncharacterized bands (*). Membrane enriched extracts (30 µg lysate) of PC-12 (Lane 1), PC-12 differentiated to neurons (Lane 2), Mouse Cerebellum (Lane 3), Mouse Brain (Lane 4), Rat Brain (Lane 5), Mouse Heart (Lane 6) and Mouse Lung (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2,000 dilution) and detected by chemiluminescence with Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP (Product # A16054, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). * - There were few uncharacterized bands observed in PC-12 differentiated to neurons, Mouse Cerebellum and Mouse Brain.

NEFL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of NEFL was observed in PC-12 differentiated to neurons in comparison to PC-12 undifferentiated cells. Relative expression was also observed in Mouse Cerebellum, Mouse Brain and Rat Brain in comparison to Mouse Heart and Mouse Lung using Anti-NEFL Polyclonal antibody (Product # PA1-10000) in Western Blot. {RE}

Product Details

PA1-10000

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:5,000	-
Immunohistochemistry (IHC)	1:2,000	-
Immunocytochemistry (ICC/IF)	1:2,000	View 1 publication 1 publication

Product Specifications
Species Reactivity	Bovine, Human, Mouse, Pig, Rat
Published species	Human
Host/Isotype	Chicken / IgY
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant human NF-L protein
Conjugate	Unconjugated
Form	Liquid
Purification	PEG precipitation
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_1077181

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enzymatically crosslinked gelatin-laminin hydrogels for applications in neuromuscular tissue engineering.

Biomaterials science

Besser RR,Bowles AC,Alassaf A,Carbonero D,Claure I,Jones E,Reda J,Wubker L,Batchelor W,Ziebarth N,Silvera R,Khan A,Maciel R,Saporta M,Agarwal A

PA1-10000 was used in Immunocytochemistry to report a water-soluble and non-toxic method to incorporate additional extracellular matrix proteins into gelatin hydrogels, while obviating the use of chemical crosslinkers such as glutaraldehyde.

Tue Jan 21 00:00:00 EST 2020

Bioinformatics

Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110

UniProt ID: (Bovine) P02548, (Human) P07196, (Mouse) P08551, (Rat) P19527

Entrez Gene ID: (Bovine) 281348, (Human) 4747, (Pig) 100521224, (Mouse) 18039, (Rat) 83613

Function(s)