Invitrogen

NEFL Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (28) NEFL antibodies

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Datasheet

Questions & Answers

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NEFL Antibody in Western Blot (WB) — Western blot was performed using Anti-NEFL Polyclonal Antibody (Product # PA1-10000) and a 68 kDa band corresponding to NEFL was observed in PC-12 differentiated to neurons, Mouse Cerebellum, Mouse Brain and Rat Brain but not in PC-12, Mouse Heart and Mouse Lung along with few uncharacterized bands (*). Membrane enriched extracts (30 µg lysate) of PC-12 (Lane 1), PC-12 differentiated to neurons (Lane 2), Mouse Cerebellum (Lane 3), Mouse Brain (Lane 4), Rat Brain (Lane 5), Mouse Heart (Lane 6) and Mouse Lung (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2,000 dilution) and detected by chemiluminescence with Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP (Product # A16054, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). * - There were few uncharacterized bands observed in PC-12 differentiated to neurons, Mouse Cerebellum and Mouse Brain.

NEFL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NEFL in rat cerebellum. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained with an NEFL polyclonal antibody (Product # PA1-10000) at a dilution of 1:2,000 in green, and costained with NeuN/Fox3 monoclonal antibody at a dilution of 1:5,000 in red. The NEFL polyclonal antibody (Product # PA1-10000) antibody labels perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while the NeuN/Fox3 antibody stains the nuclei and proximal cytoplasm of neurons.

NEFL Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of NEFL was observed in PC-12 differentiated to neurons in comparison to PC-12 undifferentiated cells. Relative expression was also observed in Mouse Cerebellum, Mouse Brain and Rat Brain in comparison to Mouse Heart and Mouse Lung using Anti-NEFL Polyclonal antibody (Product # PA1-10000) in Western Blot. {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2000-1:5000

Immunohistochemistry (IHC)

1:2000

Immunocytochemistry (ICC/IF)

1:2000

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human NF-L protein

Conjugate

Unconjugated

Form

Liquid

Purification

PEG precipitation

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_1077181

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110

UniProt ID: (Human) B9ZVN2, (Mouse) P08551, (Rat) P19527

Entrez Gene ID: (Bovine) 281348, (Human) 4747, (Pig) 100521224, (Mouse) 18039, (Rat) 83613

Molecular Function: cytoskeletal protein intermediate filament structural protein