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Invitrogen
NEFL Polyclonal Antibody
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FIGURE: 1 / 5
NEFL Antibody (PA3-16719) in ICC/IF
Immunocytochemistry analysis of NEFL in mixed neuron/glia cultures from newborn rat brain. Samples were incubated in NEFL polyclonal antibody (Product # PA3-16719). Antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains... View More
Product Details
PA3-16719
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Native Neurofilament Light protein purified from pig spinal cord.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.035% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: antigen standard for NEFL (transient overexpression lysate), rat cerebellum.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; N-FL; NEF3; NEFL; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110
Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110
UniProt ID: (Bovine) P02548, (Human) P07196, (Mouse) P08551, (Rat) P19527
Entrez Gene ID: (Bovine) 281348, (Human) 4747, (Pig) 100521224, (Mouse) 18039, (Rat) 83613
structural constituent of cytoskeleton
protein C-terminus binding
protein binding, bridging
identical protein binding
structural constituent of postsynaptic intermediate filament cytoskeleton
Ras guanyl-nucleotide exchange factor activity
protein binding
protein domain specific binding
phospholipase binding
protein heterodimerization activity
structural molecule activity
microtubule cytoskeleton organization
anterograde axonal transport
retrograde axonal transport
peripheral nervous system axon regeneration
axonal transport of mitochondrion
regulation of axon diameter
neurofilament bundle assembly
locomotion
negative regulation of neuron apoptotic process
intermediate filament organization
intermediate filament bundle assembly
neuron projection morphogenesis
positive regulation of axonogenesis
neuromuscular process controlling balance
neurofilament cytoskeleton organization
synapse maturation
postsynaptic intermediate filament cytoskeleton organization
MAPK cascade
response to toxic substance
spinal cord development
hippocampus development
cerebral cortex development
response to peptide hormone
positive regulation of GTPase activity
intermediate filament polymerization or depolymerization
protein polymerization
response to corticosterone
response to sodium arsenite
response to acrylamide
axon development
response to organic substance
response to organonitrogen compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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