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Invitrogen
NEFM Polyclonal Antibody
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FIGURE: 1 / 10
NEFM Antibody (PA1-10001) in ICC/IF
Immunofluorescence analysis of NF-M was performed using 70% confluent log phase HEK-293 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. HEK-293 cells were labeled with NF-M Chicken Polyclonal Antibody (Product # PA1-10001) at 1:1000 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-... View More
Product Details
PA1-10001
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Horse,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-10001 was made against a recombinant fusion protein of E. coli TrpE fused to the C-terminus of rat NF-M, amino acids 677-845.
Target Information
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 160 kDa neurofilament protein; major neuronal intermediate filament subunit; NEFM; Neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; Neurofilament medium polypeptide; neurofilament medium tail domain; neurofilament protein M; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-M; neuronal intermediate filament; NF-M; NF-M c-terminus; NF-M protein; NMC
Gene Aliases: NEF3; NEFM; NF-M; NF160; NF165; NFM
UniProt ID: (Human) P07197, (Chicken) P16053, (Mouse) P08553, (Rat) P12839
Entrez Gene ID: (Horse) 100054129, (Bovine) 281347, (Human) 4741, (Pig) 100625407, (Chicken) 396206, (Mouse) 18040, (Rat) 24588
neurofilament bundle assembly
axon development
mitophagy in response to mitochondrial depolarization
microtubule cytoskeleton organization
axo-dendritic transport
regulation of axon diameter
intermediate filament cytoskeleton organization
intermediate filament polymerization or depolymerization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
spinal cord development
axon regeneration
cellular response to oxidative stress
cellular response to estradiol stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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