Immunofluorescent analysis of NEFM in sections of rat cerebral cortex using a NEFM polyclonal antibody (Product # PA1-32348) (red), which reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them. The green channel shows staining with a monoclonal antibody to the beta-adrendergic receptor kinase 1.
|Tested species reactivity||Avian, Bovine, Cat, Human, Mammal, Mouse, Pig, Reptile, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant C-terminal segment of rat NF-M|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32348 detects NEFM from rat samples.
PA1-32348 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), Western blot applications.
The PA1-32348 immunogen is: Recombinant C-terminal segment of rat NF-M
neuronal intermediate filament protein; important component of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.