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Immunofluorescent analysis of NEFM in sections of rat cerebral cortex using a NEFM polyclonal antibody (Product # PA1-32348) (red), which reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them. The green channel shows staining with a monoclonal antibody to the beta-adrendergic receptor kinase 1.
|Tested species reactivity||Mouse, Pig, Reptile, Rat, Avian, Bovine, Cat, Human, Mammals|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant C-terminal segment of rat NF-M|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32348 detects NEFM from rat samples.
PA1-32348 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), Western blot applications.
The PA1-32348 immunogen is: Recombinant C-terminal segment of rat NF-M
neuronal intermediate filament protein; important component of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
160 kDa neurofilament protein; major neuronal intermediate filament subunit; Nef3; neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium polypeptide; neurofilament protein M; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-M; NF-M; NF-M c-terminus; NF-M protein; Nfm
BOS_9093; NEF3; NEFM; NF-M; NF160; NF165; NFM