Immunofluorescent analysis of the neurofilament medium chain in paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at room temperature. Tissues were then probed with a neurofilament light chain monoclonal antibody (Product # 34-1000) in 0.3% BSA at a dilution of 1:50 for 1 hour at 37°C. Tissues were then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei (blue) were stained with DAPI. Images were taken at 40X magnification.
|Tested species reactivity||Chicken, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rat, Rodent, Sheep, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||1:50 - 1:100|
|Immunofluorescence (IF)||1:50 - 1:100|
|Immunohistochemistry (IHC)||1:20 - 1:100|
|Western Blot (WB)||0.5-2 ug/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-M is the medium molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 160 kDa.
Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. NF-H polyclonal antibody can be used to identify neurons and their processes in tissue sections and in tissue culture and the antibody can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Temporal Sequence of Autolysis in the Cerebellar Cortex of the Mouse.
34-1000 was used in immunohistochemistry - paraffin section to study the cerebellar cortex of the mouse for the temporal sequence of autolysis
|Finnie JW,Blumbergs PC,Manavis J||Journal of comparative pathology (154:323)||2016|
Selective induction of ultrastructural (neurofilament) compaction in axons by means of a new head-injury apparatus.
34-1000 was used in immunohistochemistry - paraffin section to develop a new weight-drop head-injury apparatus and assess the treated rats
|Pál J,Tóth Z,Farkas O,Kellényi L,Dóczi T,Gallyas F||Journal of neuroscience methods (153:283)||2006|
Temporal assessment of traumatic axonal injury in the rat corpus callosum and optic chiasm.
34-1000 was used in immunohistochemistry to perform a time course to study impaired axoplasmic transport and neurofilament compaction.
|Zakaria N,Kallakuri S,Bandaru S,Cavanaugh JM||Brain research (1467:81)||2012|
|Corticotropin-releasing factor 2 receptor localization in skeletal muscle.||Samuelsson S,Lange JS,Hinkle RT,Tarnopolsky M,Isfort RJ||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (52:967)||2004|
Neuroaxonal dystrophy in Australian Merino lambs.
34-1000 was used in immunohistochemistry (paraffin) to describe the clinicopathological changes in the second occurrence of neuroaxonal dystrophy reported in Merino lambs.
|Kessell AE,Finnie JW,Blumbergs PC,Manavis J,Jerrett IV||Journal of comparative pathology (147:62)||2012|
A comparison of differentiation protocols for RGC-5 cells.
34-1000 was used in western blot to treat RGC-5 cells with known differentiating agents and determine which RGC or neuronal markers are expressed
|Wood JP,Chidlow G,Tran T,Crowston JG,Casson RJ||Investigative ophthalmology and visual science (51:3774)||2010|
Neural differentiation of mouse embryonic stem cells in vitro and after transplantation into eyes of mutant mice with rapid retinal degeneration.
34-1000 was used in immunocytochemistry and immunohistochemistry - frozen section to examine the differentiation of embryonic stem cells after their transplantation into the eyes of mice with hereditary retinal degeneration
|Meyer JS,Katz ML,Maruniak JA,Kirk MD||Brain research (1014:131)||2004|