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Immunofluorescent analysis of the neurofilament medium chain in paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at room temperature. Tissues were then probed with a neurofilament light chain monoclonal antibody (Product # 34-1000) in 0.3% BSA at a dilution of 1:50 for 1 hour at 37°C. Tissues were then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei (blue) were stained with DAPI. Images were taken at 40X magnification.
|Tested species reactivity||Chicken, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rat, Rodent, Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||1:50 - 1:100|
|Immunofluorescence (IF)||1:50 - 1:100|
|Immunohistochemistry (IHC)||1:20 - 1:100|
|Western Blot (WB)||0.5-2 ug/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-M is the medium molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 160 kDa.
Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. NF-H polyclonal antibody can be used to identify neurons and their processes in tissue sections and in tissue culture and the antibody can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Temporal assessment of traumatic axonal injury in the rat corpus callosum and optic chiasm.
34-1000 was used in immunohistochemistry to perform a time course to study impaired axoplasmic transport and neurofilament compaction.
|Zakaria N,Kallakuri S,Bandaru S,Cavanaugh JM||Brain research (1467:81)||2012|
|Corticotropin-releasing factor 2 receptor localization in skeletal muscle.||Samuelsson S,Lange JS,Hinkle RT,Tarnopolsky M,Isfort RJ||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (52:967)||2004|
160 kDa neurofilament protein; medium polypeptide 150kDa; NEF3; neurofilament; Neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; Neurofilament medium polypeptide; neurofilament protein M; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-M; NF-M; NF-M c-terminus; NF-M protein; NFM
NEF3; NEFM; NF-M; NF160; NF165; NFM