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Invitrogen
NEFM Monoclonal Antibody (RNF 406)
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Product Details
OMA1-06111
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Guinea pig,
Hamster,
Human,
Non-human primate,
Rabbit,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
RNF 406
Immunogen
Neurofilament preparation of calf brain tissue
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
OMA1-06111 specifically detects the phosphorylated 160 kDa neurofilament from human, guinea pig, hamster, non-human primate, rabbit and xenopus tissues.
OMA1-06111 has been successfully used in Western blot and immunohistochemistry (frozen) procedures. By Western blot, this antibody detects an ~160 kDa protein representing the phosphorylated form of the 160 kDa neurofilament in human brain extract.
OMA1-06111 antigen is cytoskeletal preparation of calf brain tissue.
Target Information
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 160 kDa neurofilament protein; major neuronal intermediate filament subunit; medium neurofilament subunit; middle molecular weight neurofilament protein NF-M(2); NEFM; Neurofilament 3; neurofilament 3, medium; neurofilament M subunit; Neurofilament medium polypeptide; neurofilament protein M; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neuronal intermediate filament; NF-M; NMC
Gene Aliases: nef-m; NEF3; NEFM; nefm-a; nefm-b; NF-M; NFM; XELAEV_18020229mg
UniProt ID: (Human) P07197, (Rat) P12839
neurofilament bundle assembly
axon development
mitophagy in response to mitochondrial depolarization
microtubule cytoskeleton organization
axo-dendritic transport
spinal cord development
axon regeneration
regulation of axon diameter
cellular response to oxidative stress
intermediate filament cytoskeleton organization
intermediate filament polymerization or depolymerization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
cellular response to estradiol stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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