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Invitrogen
NEIL3 Polyclonal Antibody
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Product Details
PA1-4304
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide: L(164) R A E S E V K K Q K G R M L G(177)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-4304 detects the human NEI3 protein in human samples.
PA1-4304 has been successfully used in Western blot procedures. By Western blot this antibody detects a ~68 kDa protein representing NEI3 in human testis lysate.
PA1-4304 immunizing peptide corresponds to amino acid residues 164-177 of the human NEI3 protein. There is 85% sequence identity to mouse and rat.
Target Information
DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA glycosylase FPG2; DNA glycosylase hFPG2; DNA glycosylase/AP lyase Neil3; Endonuclease 8-like 3; Endonuclease VIII-like 3; FLJ10858; nei endonuclease VIII-like 3; Nei-like protein 3; unnamed protein product; zinc finger, GRF-type containing 3
Gene Aliases: FGP2; FPG2; hFPG2; hNEI3; NEI3; NEIL3; ZGRF3
UniProt ID: (Human) Q8TAT5
Entrez Gene ID: (Human) 55247
bubble DNA binding
nucleic acid binding
DNA binding
damaged DNA binding
double-stranded DNA binding
single-stranded DNA binding
catalytic activity
DNA-(apurinic or apyrimidinic site) lyase activity
zinc ion binding
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing N-glycosyl compounds
lyase activity
DNA N-glycosylase activity
metal ion binding
class I DNA-(apurinic or apyrimidinic site) endonuclease activity
MCM complex binding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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