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Invitrogen
NEIL3 Polyclonal Antibody
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FIGURE: 1 / 2
NEIL3 Antibody (PA5-50028) in IHC (P)
Immunohistochemistry analysis of NEIL3 on paraffin-embedded human breast cancer tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the NEIL3 Polyclonal Antibody (Product # PA5-50028) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was the... View More
Product Details
PA5-50028
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthesized peptide derived from internal of human NEIL3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 30% glycerol, 0.87% NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects endogenous levels of total NEIL3 protein.
Target Information
DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA glycosylase FPG2; DNA glycosylase hFPG2; DNA glycosylase/AP lyase Neil3; Endonuclease 8-like 3; Endonuclease VIII-like 3; FLJ10858; nei endonuclease VIII-like 3; nei like 3; Nei-like protein 3; unnamed protein product; zinc finger, GRF-type containing 3
Gene Aliases: AI449477; BC034753; C85903; FGP2; FPG2; hFPG2; hNEI3; NEI3; NEIL3; RGD1310562; ZGRF3
UniProt ID: (Human) Q8TAT5, (Mouse) Q8K203
Entrez Gene ID: (Human) 55247, (Mouse) 234258, (Rat) 290729
bubble DNA binding
nucleic acid binding
DNA binding
damaged DNA binding
double-stranded DNA binding
single-stranded DNA binding
catalytic activity
DNA-(apurinic or apyrimidinic site) lyase activity
zinc ion binding
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing N-glycosyl compounds
lyase activity
DNA N-glycosylase activity
metal ion binding
class I DNA-(apurinic or apyrimidinic site) endonuclease activity
MCM complex binding
purine-specific mismatch base pair DNA N-glycosylase activity
uracil DNA N-glycosylase activity
single-strand selective uracil DNA N-glycosylase activity
DNA-3-methylbase glycosylase activity
nicotinamide riboside hydrolase activity
nicotinic acid riboside hydrolase activity
deoxyribonucleoside 5'-monophosphate N-glycosidase activity
DNA glycosylase
single strand break repair
DNA repair
base-excision repair
base-excision repair, AP site formation
cellular response to DNA damage stimulus
interstrand cross-link repair
depurination
DNA metabolic process
nucleotide-excision repair
metabolic process
nucleic acid phosphodiester bond hydrolysis
DNA catabolic process, endonucleolytic
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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